Literature summary for 2.3.2.12 extracted from

Boeth, D.; Steiner, E.M.; Stadler, D.; Lindqvist, Y.; Schnell, R.; Schneider, G.
Structure of LdtMt2, an L,D-transpeptidase from Mycobacterium tuberculosis (2013), Acta Crystallogr. Sect. D, 69, 432-441.

Search for more literature for 2.3.2.12

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M bis-Tris-HCl pH 6.5, 25% (w/v) PEG 3350	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptidyl-tRNA1 + aminoacyl-tRNA2	Mycobacterium tuberculosis	-	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?
peptidyl-tRNA1 + aminoacyl-tRNA2	Mycobacterium tuberculosis CDC 1551	-	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	O53223	-	-
Mycobacterium tuberculosis CDC 1551	O53223	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptidyl-tRNA1 + aminoacyl-tRNA2	-	Mycobacterium tuberculosis	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?
peptidyl-tRNA1 + aminoacyl-tRNA2	-	Mycobacterium tuberculosis CDC 1551	tRNA1 + peptidyl(aminoacyl-tRNA2)	-	?

Synonyms

Synonyms	Comment	Organism
L,D-transpeptidase	-	Mycobacterium tuberculosis
LdtMt2	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)