Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chloramphenicol | competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin | Escherichia coli | |
Gly-chloramphenicol | competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin | Escherichia coli | |
Gly-Phe-chloramphenicol | competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin | Escherichia coli | |
L-Phe-chloramphenicol | competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin | Escherichia coli | |
L-Phe-Gly-chloramphenicol | competitive inhibition with acetylphenylalanyl-tRNA-polyU-ribosome complex, newly formed complex is inactive towards puromycin | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
ribosome | additionally factors washable from ribosomes | Escherichia coli | 5840 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
B cells, frozen | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
AcPhe-tRNA + puromycin | AcPhe-tRNA-polyU-ribosome complex | Escherichia coli | tRNA + AcPhe-puromycin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
enzyme assay | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
L-Phe-Gly-chloramphenicol | - |
Escherichia coli | |
0.0058 | - |
Gly-chloramphenicol | - |
Escherichia coli | |
0.007 | - |
chloramphenicol | - |
Escherichia coli | |
0.02 | - |
L-Phe-chloramphenicol | - |
Escherichia coli | |
0.09 | - |
Gly-Phe-chloramphenicol | - |
Escherichia coli |