Cloned (Comment) | Organism |
---|---|
enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
D579A | the mutant shows decreased activity (3%) compared to the wild type enzyme | Pseudomonas aeruginosa |
D579N | the mutant shows decreased activity (5%) compared to the wild type enzyme | Pseudomonas aeruginosa |
D710A | the mutant has 26% of wild type activity | Pseudomonas aeruginosa |
D710N | the mutant has 30% of wild type activity | Pseudomonas aeruginosa |
D765A | inactive | Pseudomonas aeruginosa |
D765N | inactive | Pseudomonas aeruginosa |
E657D | the mutant shows decreased activity (4%) compared to the wild type enzyme | Pseudomonas aeruginosa |
E657Q | the mutant has 21% of wild type activity | Pseudomonas aeruginosa |
E658D | the mutant has 28% of wild type activity | Pseudomonas aeruginosa |
E658Q | the mutant has 19% of wild type activity | Pseudomonas aeruginosa |
E720Q | the mutant has 10% of wild type activity | Pseudomonas aeruginosa |
K580Q | inactive | Pseudomonas aeruginosa |
K580S | inactive | Pseudomonas aeruginosa |
K654Q | the mutant has 65% of wild type activity | Pseudomonas aeruginosa |
K654S | the mutant has 46% of wild type activity | Pseudomonas aeruginosa |
K840Q | inactive | Pseudomonas aeruginosa |
K840S | inactive | Pseudomonas aeruginosa |
K842Q | the mutant has wild type activity | Pseudomonas aeruginosa |
K842S | the mutant has 50% of wild type activity | Pseudomonas aeruginosa |
M778A | the mutant has 50% of wild type activity | Pseudomonas aeruginosa |
R768Q | inactive | Pseudomonas aeruginosa |
R768S | inactive | Pseudomonas aeruginosa |
R837Q | the mutant has wild type activity | Pseudomonas aeruginosa |
R837S | the mutant has 65% of wild type activity | Pseudomonas aeruginosa |
S709A | the mutant has 5% of wild type activity | Pseudomonas aeruginosa |
S709N | the mutant has 17% of wild type activity | Pseudomonas aeruginosa |
S724A | the mutant shows decreased activity (7%) compared to the wild type enzyme | Pseudomonas aeruginosa |
S724N | inactive | Pseudomonas aeruginosa |
S763A | the mutant has 23% of wild type activity | Pseudomonas aeruginosa |
S763N | the mutant has 11% of wild type activity | Pseudomonas aeruginosa |
Y635A | inactive | Pseudomonas aeruginosa |
Y732A | the mutant shows decreased activity (3%) compared to the wild type enzyme | Pseudomonas aeruginosa |
Y831A | inactive | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 20% residual activity at 20 mM | Pseudomonas aeruginosa | |
additional information | in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a transmembrane protein which is located in the inner membrane | Pseudomonas aeruginosa | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme catalysis is not dependent on metal ions | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
gel filtration | Pseudomonas aeruginosa |
38000 | - |
1 * 38000, SDS-PAGE | Pseudomonas aeruginosa |
38720 | - |
1 * 38720, calculated from amino acid sequence | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol) | 20% activity compared to phosphatidylglycerol | Pseudomonas aeruginosa | ? | - |
? | |
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol) | 100% activity compared to phosphatidylglycerol | Pseudomonas aeruginosa | ? | - |
? | |
L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol) | 20% activity compared to phosphatidylglycerol | Pseudomonas aeruginosa | ? | - |
? | |
L-alanyl-tRNAAla + phosphatidylglycerol | 100% activity | Pseudomonas aeruginosa | tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol | - |
? | |
microhelix A + phosphatidylglycerol | 90% activity compared to L-alanyl-tRNA | Pseudomonas aeruginosa | ? | - |
? | |
microhelix B + phosphatidylglycerol | 40% activity compared to L-alanyl-tRNA | Pseudomonas aeruginosa | ? | - |
? | |
microhelix C + phosphatidylglycerol | - |
Pseudomonas aeruginosa | ? | - |
? | |
microhelix D + phosphatidylglycerol | - |
Pseudomonas aeruginosa | ? | - |
? | |
microhelix E + phosphatidylglycerol | 90% activity compared to L-alanyl-tRNA | Pseudomonas aeruginosa | ? | - |
? | |
additional information | 1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 38000, SDS-PAGE | Pseudomonas aeruginosa |
monomer | 1 * 38720, calculated from amino acid sequence | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
A-PGS | - |
Pseudomonas aeruginosa |
alanyl-phosphatidylglycerol synthase | - |
Pseudomonas aeruginosa |