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Literature summary for 2.3.2.11 extracted from

  • Hebecker, S.; Arendt, W.; Heinemann, I.U.; Tiefenau, J.H.; Nimtz, M.; Rohde, M.; Soell, D.; Moser, J.
    Alanyl-phosphatidylglycerol synthase: mechanism of substrate recognition during tRNA-dependent lipid modification in Pseudomonas aeruginosa (2011), Mol. Microbiol., 80, 935-950.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.2.11

Cloned(Commentary)

Cloned (Comment) Organism
enzymatically active residues 543-881 N-terminally fused to glutathione S-transferase are expressed in Escherichia coli BL21(lambdaDE3) cells Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
D579A the mutant shows decreased activity (3%) compared to the wild type enzyme Pseudomonas aeruginosa
D579N the mutant shows decreased activity (5%) compared to the wild type enzyme Pseudomonas aeruginosa
D710A the mutant has 26% of wild type activity Pseudomonas aeruginosa
D710N the mutant has 30% of wild type activity Pseudomonas aeruginosa
D765A inactive Pseudomonas aeruginosa
D765N inactive Pseudomonas aeruginosa
E657D the mutant shows decreased activity (4%) compared to the wild type enzyme Pseudomonas aeruginosa
E657Q the mutant has 21% of wild type activity Pseudomonas aeruginosa
E658D the mutant has 28% of wild type activity Pseudomonas aeruginosa
E658Q the mutant has 19% of wild type activity Pseudomonas aeruginosa
E720Q the mutant has 10% of wild type activity Pseudomonas aeruginosa
K580Q inactive Pseudomonas aeruginosa
K580S inactive Pseudomonas aeruginosa
K654Q the mutant has 65% of wild type activity Pseudomonas aeruginosa
K654S the mutant has 46% of wild type activity Pseudomonas aeruginosa
K840Q inactive Pseudomonas aeruginosa
K840S inactive Pseudomonas aeruginosa
K842Q the mutant has wild type activity Pseudomonas aeruginosa
K842S the mutant has 50% of wild type activity Pseudomonas aeruginosa
M778A the mutant has 50% of wild type activity Pseudomonas aeruginosa
R768Q inactive Pseudomonas aeruginosa
R768S inactive Pseudomonas aeruginosa
R837Q the mutant has wild type activity Pseudomonas aeruginosa
R837S the mutant has 65% of wild type activity Pseudomonas aeruginosa
S709A the mutant has 5% of wild type activity Pseudomonas aeruginosa
S709N the mutant has 17% of wild type activity Pseudomonas aeruginosa
S724A the mutant shows decreased activity (7%) compared to the wild type enzyme Pseudomonas aeruginosa
S724N inactive Pseudomonas aeruginosa
S763A the mutant has 23% of wild type activity Pseudomonas aeruginosa
S763N the mutant has 11% of wild type activity Pseudomonas aeruginosa
Y635A inactive Pseudomonas aeruginosa
Y732A the mutant shows decreased activity (3%) compared to the wild type enzyme Pseudomonas aeruginosa
Y831A inactive Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 20% residual activity at 20 mM Pseudomonas aeruginosa
additional information in the presence of up to 20 mM EDTA or up to 5 mM 1,10-phenanthroline no significant inactivation is observed Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a transmembrane protein which is located in the inner membrane Pseudomonas aeruginosa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme catalysis is not dependent on metal ions Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 gel filtration Pseudomonas aeruginosa
38000
-
 1 * 38000, SDS-PAGE Pseudomonas aeruginosa
38720
-
 1 * 38720, calculated from amino acid sequence Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alanyl-tRNA + 1,2-dicaproyl-sn-glycero-3-phospho-(1'-rac-glycerol) 20% activity compared to phosphatidylglycerol Pseudomonas aeruginosa ?
-
 ?
L-alanyl-tRNA + 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol) 100% activity compared to phosphatidylglycerol Pseudomonas aeruginosa ?
-
 ?
L-alanyl-tRNA + 1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol) 20% activity compared to phosphatidylglycerol Pseudomonas aeruginosa ?
-
 ?
L-alanyl-tRNAAla + phosphatidylglycerol 100% activity Pseudomonas aeruginosa tRNAAla + 3-O-L-alanyl-1-O-phosphatidylglycerol
-
 ?
microhelix A + phosphatidylglycerol 90% activity compared to L-alanyl-tRNA Pseudomonas aeruginosa ?
-
 ?
microhelix B + phosphatidylglycerol 40% activity compared to L-alanyl-tRNA Pseudomonas aeruginosa ?
-
 ?
microhelix C + phosphatidylglycerol
-
 Pseudomonas aeruginosa ?
-
 ?
microhelix D + phosphatidylglycerol
-
 Pseudomonas aeruginosa ?
-
 ?
microhelix E + phosphatidylglycerol 90% activity compared to L-alanyl-tRNA Pseudomonas aeruginosa ?
-
 ?
additional information 1,2-diplamitoyl-sn-glycero-3-phospho(ethylene glycol), microhelix F, N-acetylalanyl-alanyl-tRNA, lactyl-alanyl-tRNA and phenylalanyl-alanyl-tRNA are no substrates Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Pseudomonas aeruginosa
monomer 1 * 38720, calculated from amino acid sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
A-PGS
-
 Pseudomonas aeruginosa
alanyl-phosphatidylglycerol synthase
-
 Pseudomonas aeruginosa