BRENDA - Enzyme Database
show all sequences of 2.3.1.B5

Alcoholytic cleavage of polyhydroxyalkanoate chains by class IV synthases induced by endogenous and exogenous ethanol

Hyakutake, M.; Tomizawa, S.; Mizuno, K.; Abe, H.; Tsuge, T.; Appl. Environ. Microbiol. 80, 1421-1429 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Bacillus cereus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus cereus
D2Z0B8 and D2Z0B6
D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
-
Bacillus cereus YB-4
D2Z0B8 and D2Z0B6
D2Z0B8 i.e. subunit PhaC, D2Z0B6 i.e. subunit PhaR
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
735496
Bacillus cereus
[(R)-3-hydroxybutanoate](n+1) + CoA
-
-
-
?
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
735496
Bacillus cereus YB-4
[(R)-3-hydroxybutanoate](n+1) + CoA
-
-
-
?
additional information
enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol
735496
Bacillus cereus
?
-
-
-
-
additional information
enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol
735496
Bacillus cereus YB-4
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
735496
Bacillus cereus
[(R)-3-hydroxybutanoate](n+1) + CoA
-
-
-
?
(R)-3-hydroxybutyryl-CoA + [(R)-3-hydroxybutanoate]n
-
735496
Bacillus cereus YB-4
[(R)-3-hydroxybutanoate](n+1) + CoA
-
-
-
?
additional information
enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol
735496
Bacillus cereus
?
-
-
-
-
additional information
enzyme shows alcoholytic cleavage of PHA chains induced by endogenous ethanol
735496
Bacillus cereus YB-4
?
-
-
-
-
General Information
General Information
Commentary
Organism
physiological function
recombinant Escherichia coli expressing PHA synthase from Bacillus cereus shows a reduction of the molecular weight of PHA produced during the stationary phase of growth. Its carboxy end structure is capped by ethanol, as the result of alcoholytic cleavage of PHA chains by PhaRC induced by endogenous ethanol. This scission reaction is also induced by exogenous ethanol in both in vivo and in vitro assays. In addition, PhaRC has alcoholysis activity for PHA chains synthesized by other synthases
Bacillus cereus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
recombinant Escherichia coli expressing PHA synthase from Bacillus cereus shows a reduction of the molecular weight of PHA produced during the stationary phase of growth. Its carboxy end structure is capped by ethanol, as the result of alcoholytic cleavage of PHA chains by PhaRC induced by endogenous ethanol. This scission reaction is also induced by exogenous ethanol in both in vivo and in vitro assays. In addition, PhaRC has alcoholysis activity for PHA chains synthesized by other synthases
Bacillus cereus
Other publictions for EC 2.3.1.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735919
Klask
-
Heterologous expression of var ...
Rhodospirillum rubrum
Chem. Biochem. Eng. Q.
29
75-85
2015
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1
1
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737152
Hyakutake
-
Carboxy-terminal modification ...
Bacillus cereus, Bacillus cereus YB-4
Polym. Degrad. Stab.
117
90-96
2015
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1
1
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735496
Hyakutake
Alcoholytic cleavage of polyhy ...
Bacillus cereus, Bacillus cereus YB-4
Appl. Environ. Microbiol.
80
1421-1429
2014
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1
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4
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1
1
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736277
Gowda
-
Poly(3)hydroxybutyrate (PHB) p ...
Bacillus thuringiensis, Bacillus thuringiensis IAM 12077
Int. J. Pharm. Bio Sci.
4
B794-B802
2013
1
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2
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-
-
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719032
Tomizawa
Molecular weight change of pol ...
Bacillus cereus, Bacillus cereus YB-4, Bacillus megaterium, Bacillus megaterium NBRC15308
Biomacromolecules
12
2660-2666
2011
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2
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2
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6
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4
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10
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2
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6
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10
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719746
Tan
Cloning and characterization o ...
Pseudomonas sp., Pseudomonas sp. USM4-55
J. Basic Microbiol.
50
179-189
2010
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1
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1
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5
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1
1
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721109
Kek
-
Heterologous expression of Cup ...
Cupriavidus sp., Cupriavidus sp. USMAA2-4
World J. Microbiol. Biotechnol.
26
1595-1603
2010
-
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1
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4
-
2
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1
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4
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1
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4
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1
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4
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1
1
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684934
Dai
Poly-3-hydroxybutyrate synthas ...
Escherichia coli
Biochem. Biophys. Res. Commun.
374
485-489
2008
-
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1
1
1
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1
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1
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688122
Sharma
Studies on poly-beta-hydroxybu ...
Desmonostoc muscorum
J. Gen. Appl. Microbiol.
52
209-214
2006
1
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1
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3
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1
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1
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684663
Hezayen
Biochemical and enzymological ...
Halopiger aswanensis, Halopiger aswanensis DSM 13151
Arch. Biochem. Biophys.
403
284-291
2002
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1
1
1
2
1
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2
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1
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1
1
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1
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1
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688851
Qi
Polyhydroxybutyrate biosynthes ...
Caulobacter vibrioides
Microbiology
147
3353-3358
2001
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1
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1
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689122
Sim
PHA synthase activity controls ...
Cupriavidus necator
Nat. Biotechnol.
15
63-67
1997
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1
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687271
Wodzinska
-
Polyhydroxybutyrate synthase: ...
Cupriavidus necator
J. Am. Chem. Soc.
118
6319-6320
1996
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