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Literature summary for 2.3.1.B43 extracted from

  • Yokoyama, T.; Takayama, Y.; Mizuguchi, M.; Nabeshima, Y.; Kusaka, K.
    SIRT5 mutants reveal the role of conserved asparagine and glutamine residues in the NAD+-binding pocket (2024), FEBS Lett., 598, 2269-2280.
    View publication on PubMed
Search for more literature for 2.3.1.B43

Cloned(Commentary)

Cloned (Comment) Organism
expression as an N-terminal His-tagged protein in the Escherichia coli BL21(DE3) Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor-diffusion method at 20°C Homo sapiens

Protein Variants

Protein Variants Comment Organism
H158M catalytic efficiency (kcat/Km) of the mutant decreases to 0.0021 times that of the wild-type SIRT5 Homo sapiens
N141V catalytic efficiency (kcat/Km) of the mutant decreases to 0.0038 times that of the wild-type SIRT5 Homo sapiens
Q140L catalytic efficiency (kcat/Km) of the mutant decreases to 0.02 times that of the wild-type SIRT5 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0165
-
 Fluor de Lys-succinyl pH 7.4, 37°C, wild-type enzyme Homo sapiens
0.033
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme H158M Homo sapiens
0.0613
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme Q140L Homo sapiens
0.18
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme N141V Homo sapiens
0.449
-
 NAD+ pH 7.4, 37°C, wild-type enzyme Homo sapiens
0.486
-
 NAD+ pH 7.4, 37°C, mutant enzyme H158M Homo sapiens
0.844
-
 NAD+ pH 7.4, 37°C, mutant enzyme Q140L Homo sapiens
1.38
-
 NAD+ pH 7.4, 37°C, mutant enzyme N141V Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NXA8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Fluor de Lys-succinyl + NAD+ + H2O
-
 Homo sapiens Fluor de Lys + 2''O-succinyl-ADP-D-ribose + nicotinamide
-
 ?
[histone H3]-N6-succinyl-L-lysine + NAD+ + H2O
-
 Homo sapiens [histone H3]-L-lysine + 2''-O-succinyl-ADP-D-ribose + nicotinamide
-
 ?

Synonyms

Synonyms Comment Organism
SIRT5
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0146
-
 NAD+ pH 7.4, 37°C, mutant enzyme H158M Homo sapiens
0.0146
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme H158M Homo sapiens
0.146
-
 NAD+ pH 7.4, 37°C, mutant enzyme N141V Homo sapiens
0.146
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme N141V Homo sapiens
0.251
-
 NAD+ pH 7.4, 37°C, mutant enzyme Q140L Homo sapiens
0.251
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme Q140L Homo sapiens
3.44
-
 NAD+ pH 7.4, 37°C, wild-type enzyme Homo sapiens
3.44
-
 Fluor de Lys-succinyl pH 7.4, 37°C, wild-type enzyme Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.03
-
 NAD+ pH 7.4, 37°C, mutant enzyme H158M Homo sapiens
0.106
-
 NAD+ pH 7.4, 37°C, mutant enzyme N141V Homo sapiens
0.297
-
 NAD+ pH 7.4, 37°C, mutant enzyme Q140L Homo sapiens
0.442
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme H158M Homo sapiens
0.811
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme N141V Homo sapiens
4.09
-
 Fluor de Lys-succinyl pH 7.4, 37°C, mutant enzyme Q140L Homo sapiens
7.66
-
 NAD+ pH 7.4, 37°C, wild-type enzyme Homo sapiens
208
-
 Fluor de Lys-succinyl pH 7.4, 37°C, wild-type enzyme Homo sapiens