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Literature summary for 2.3.1.B41 extracted from

  • Fischer, F.; Gertz, M.; Suenkel, B.; Lakshminarasimhan, M.; Schutkowski, M.; Steegborn, C.
    Sirt5 deacylation activities show differential sensitivities to nicotinamide inhibition (2012), PLoS One, 7, e45098.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.B41

Cloned(Commentary)

Cloned (Comment) Organism
-
 Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
M70R further decrease in nicotinamide sensitivity compared to wild-type Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
additional information nicotinamide insensitivity, IC50: 2.1 mM Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 SKEYFS(N6-acetyl)KQK pH 7.8, 37°C Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O30124
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information insignificant activity with SKEYFS(N6-succinyl)KQK Archaeoglobus fulgidus ?
-
 ?
NAD+ + SKEYFS(N6-acetyl)KQK
-
 Archaeoglobus fulgidus nicotinamide + SKEYFSKQK + 2'-O-acetyl-ADP-ribose
-
 ?

Synonyms

Synonyms Comment Organism
Sir2Af2
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.132
-
 SKEYFS(N6-acetyl)KQK pH 7.8, 37°C Archaeoglobus fulgidus