Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
mutant C129A, to 2 A resolution, space group P212121. The heterodimer displays a pseudo 2fold symmetry. Subunit PqsC has an active site comprised of Cys129 and His269 | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
C129A | mutation in catalytic dyad, crystallization data | Pseudomonas aeruginosa |
V299N | substitution leads to significant activity toward the deamino substrate analog benzoylacetate, suggesting that the substrate 2-aminobenzoylacetate itself supplies the asparagine-equivalent amino function that assists in catalysis | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-aminoacetophenone | pathway-inherent competitive inhibitor. EC50 of 46 microM in the in vitro assay | Pseudomonas aeruginosa | |
additional information | 2-heptyl-4(1H)-quinolone and 2-heptyl-3-hydroxy-4(1H)-quinolone as well as ,4-dihydroxyquinoline at concentrations up to 50 microM do not affect PqsBC activity | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
octanoyl-CoA | pH 8.2, temperature not specified in the publication | Pseudomonas aeruginosa | |
0.105 | - |
2-aminobenzoylacetate | pH 8.2, temperature not specified in the publication | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | the catalytic His269 residue is not involved in acyl-enzyme formation, but contributes to an acylation-dependent increase in affinity for the second substrate 2-aminobenzoylacetate. Octanoylation of Cys129 of PqsC is independent of His269 | ? | - |
? | |
octanoyl-CoA + 2-aminobenzoylacetate | Pseudomonas aeruginosa | - |
CoA + 2-heptylquinolin-4(1H)-one + CO2 + H2O | product is a signal molecule in Pseudomonas aeruginosa. The initial product is 1-(2-aminophenyl)decane-1,3-dione which spontaneously rearranges to the product under the elimination of water | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q9I4X1 | subunit PqsC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the catalytic His269 residue is not involved in acyl-enzyme formation, but contributes to an acylation-dependent increase in affinity for the second substrate 2-aminobenzoylacetate. Octanoylation of Cys129 of PqsC is independent of His269 | Pseudomonas aeruginosa | ? | - |
? | |
octanoyl-CoA + 2-aminobenzoylacetate | - |
Pseudomonas aeruginosa | CoA + 2-heptylquinolin-4(1H)-one + CO2 + H2O | product is a signal molecule in Pseudomonas aeruginosa. The initial product is 1-(2-aminophenyl)decane-1,3-dione which spontaneously rearranges to the product under the elimination of water | ? |
Synonyms | Comment | Organism |
---|---|---|
PqsBC | - |
Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
octanoyl-CoA | pH 8.2, temperature not specified in the publication | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.054 | 0.056 | 2-aminoacetophenone | pH 8.2, temperature not specified in the publication | Pseudomonas aeruginosa |