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Literature summary for 2.3.1.99 extracted from
- Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica (2010), Planta Med., 76, 1921-1926.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of His-tagged HQT in Escherichia coli | Lonicera japonica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2936 | - |
caffeoyl-CoA | pH 7.5, 30°C, recombinant enzyme | Lonicera japonica |  |
| 0.7635 | - |
quinate | pH 7.5, 30°C, recombinant enzyme | Lonicera japonica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000, about, sequence calculation | Lonicera japonica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + quinate | Lonicera japonica | - |
CoA + chlorogenic acid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lonicera japonica | D1L8A9 | - |
- |
| no activity in Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged HQT from Escherichia coli by nickel affinity chromatography | Lonicera japonica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower bud | high enzyme content | Lonicera japonica | - |
| leaf | - |
Lonicera japonica | - |
| stem | lower enzyme content | Lonicera japonica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + quinate | - |
Lonicera japonica | CoA + chlorogenic acid | - |
? | |
| caffeoyl-CoA + quinate | - |
Lonicera japonica | CoA + chlorogenic acid | i.e. chlorogenate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000, about, sequence calculation | Lonicera japonica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HQT | - |
Lonicera japonica |
| hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase | - |
Lonicera japonica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Lonicera japonica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Lonicera japonica |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | HQT is involved in one of three possible pathways of chlorogenate biosynthesis, overview | Lonicera japonica |
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