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Literature summary for 2.3.1.99 extracted from
- Engineering plants with increased levels of the antioxidant chlorogenic acid (2004), Nat. Biotechnol., 22, 746-754.
Application
| Application | Comment | Organism |
|---|---|---|
| agriculture | overexpression of the enzyme in tomato causes plants to accumulate higher levels of chlorogenic acid, with no side-effects on the levels of other soluble phenolics. Plants show improved antioxidant capacity and resistance to infection by bacterial pathogens. Tomatoes with elevated levels of chlorogenic acid could be used in foods with specific benefits for human health | Solanum lycopersicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as fusion protein with 15 amino acid S-TAG | Solanum lycopersicum |
| expression in Escherichia coli as fusion protein with S-TAG | Nicotiana tabacum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.871 | - |
quinate | - |
Nicotiana tabacum |  |
| 11.82 | - |
shikimate | - |
Nicotiana tabacum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Nicotiana tabacum | the enzyme is required for the synthesis of chlorogenic acid | ? | - |
? | |
| additional information | Solanum lycopersicum | the enzyme is required for the synthesis of chlorogenic acid | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | Q70G33 | - |
- |
| Solanum lycopersicum | Q70G32 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Nicotiana tabacum |
- |
Solanum lycopersicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.08 | - |
- |
Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| caffeoyl-CoA + quinate | - |
Nicotiana tabacum | CoA + 5-O-caffeoylquinate | - |
? | |
| caffeoyl-CoA + quinate | - |
Solanum lycopersicum | CoA + 5-O-caffeoylquinate | - |
? | |
| caffeoyl-CoA + shikimate | - |
Nicotiana tabacum | CoA + 5-O-caffeoylshikimate | - |
? | |
| caffeoyl-CoA + shikimate | - |
Solanum lycopersicum | CoA + 5-O-caffeoylshikimate | - |
? | |
| additional information | the enzyme is required for the synthesis of chlorogenic acid | Nicotiana tabacum | ? | - |
? | |
| additional information | the enzyme is required for the synthesis of chlorogenic acid | Solanum lycopersicum | ? | - |
? | |
| p-coumaroyl-CoA + quinate | - |
Nicotiana tabacum | CoA + 5-O-(p-coumaroyl)quinate | - |
? | |
| p-coumaroyl-CoA + quinate | - |
Solanum lycopersicum | CoA + 5-O-(p-coumaroyl)quinate | - |
? | |
| p-coumaroyl-CoA + shikimate | - |
Nicotiana tabacum | CoA + 5-O-(p-coumaroyl)shikimate | - |
? | |
| p-coumaroyl-CoA + shikimate | - |
Solanum lycopersicum | CoA + 5-O-(p-coumaroyl)shikimate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HQT | - |
Nicotiana tabacum |
| HQT | - |
Solanum lycopersicum |
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Release 2023.1 (January 2023)
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