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Literature summary for 2.3.1.99 extracted from

  • Hoffmann, L.; Maury, S.; Martz, F.; Geoffroy, P.; Legrand, M.
    Purification, cloning, and properties of an acyltransferase controlling shikimate and quinate ester intermediates in phenylpropanoid metabolism (2003), J. Biol. Chem., 278, 95-103.
    View publication on PubMed
Search for more literature for 2.3.1.99

Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein fused to glutathione-S-transferase is expressed in Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 caffeoyl-CoA pH 6.6, 30°C, cosubstrate: shikimate Nicotiana tabacum
0.15
-
 p-Coumaroyl-CoA pH 6.6, 30°C, cosubstrate: quinate Nicotiana tabacum
0.35
-
 feruloyl-CoA pH 6.6, 30°C, cosubstrate: shikimate Nicotiana tabacum
0.6
-
 p-Coumaroyl-CoA pH 6.6, 30°C, cosubstrate: shikimate Nicotiana tabacum
0.73
-
 caffeoyl-CoA pH 6.6, 30°C, cosubstrate: quinate Nicotiana tabacum
0.75
-
 shikimate pH 6.6, 30°C, cosubstrate: p-coumaroyl-CoA Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nicotiana tabacum the enzyme controls shikimate and quinate ester intermediates in phenylpropanoid metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q8GSM7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein fused to glutathione-S-transferase Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 decrease in absorbance at DELTA346 nM Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeoyl-CoA + quinate
-
 Nicotiana tabacum CoA + 5-O-caffeoylquinate
-
 ?
caffeoyl-CoA + shikimate caffeoyl-CoA is the most efficient acyl group donor with shikimate as substrate Nicotiana tabacum CoA + 5-O-caffeoylshikimate
-
 ?
feruloyl-CoA + shikimate feruloyl-CoA shows 10fold lower activity than caffeoyl-CoA with shikimate as substrate Nicotiana tabacum CoA + 5-O-feruloylshikimate
-
 ?
additional information the enzyme controls shikimate and quinate ester intermediates in phenylpropanoid metabolism Nicotiana tabacum ?
-
 ?
additional information no activity is detected with quinate and feruloyl-CoA or sinapoyl-CoA Nicotiana tabacum ?
-
 ?
p-coumaroyl-CoA + quinate 4-coumaroyl-CoA is the most efficient acyl group donor with quinate as substrate Nicotiana tabacum CoA + 5-O-(p-coumaroyl)quinate
-
 ?
p-coumaroyl-CoA + shikimate
-
 Nicotiana tabacum CoA + 5-O-(p-coumaroyl)shikimate
-
 ?