Any feedback?
Literature summary for 2.3.1.99 extracted from
- Partial purification and characterization of hydroxycinnamoyl CoA:transferase from apple and date fruits (1992), Phytochemistry, 31, 767-772.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Sodium diphosphate | - |
Malus sylvestris |  |
| tricine | - |
Malus sylvestris | |
| Tris/HCl | reversible | Malus sylvestris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
coumaroyl-CoA | - |
Malus sylvestris | |
| 0.009 | - |
feruloyl-CoA | - |
Malus sylvestris | |
| 0.01 | - |
caffeoyl-CoA | - |
Malus sylvestris | |
| 1.3 | - |
quinate | - |
Malus sylvestris | |
| 10 | - |
shikimate | - |
Malus sylvestris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
gel filtration | Malus sylvestris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Malus sylvestris | - |
apple, cv. Golden Delicious | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Malus sylvestris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Malus sylvestris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.37 | - |
- |
Malus sylvestris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, with 10% glycerol at least 30 days | Malus sylvestris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + quinate | best substrate | Malus sylvestris | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
| 4-coumaroyl-CoA + quinate | no substrates are citrate, malate, L-(+)-tartrate, UDP-glucose, myo-inositol, tyramine, agmatine | Malus sylvestris | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
| 4-coumaroyl-CoA + quinate | cinnamoyl-CoA cannot replace coumaroyl-CoA | Malus sylvestris | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
| 4-coumaroyl-CoA + quinate | not: glucose | Malus sylvestris | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
| caffeoyl-CoA + quinate | - |
Malus sylvestris | CoA + 5-O-caffeoylquinate | - |
? | |
| feruloyl-CoA + quinate | - |
Malus sylvestris | CoA + O-feruloylquinate | - |
? | |
| p-coumaroyl-CoA + shikimate | poor substrate | Malus sylvestris | CoA + 5-O-(p-coumaroyl)shikimate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Malus sylvestris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 5.4 | Malus sylvestris |
| 6.5 | - |
50 mM potassium phosphate buffer | Malus sylvestris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 7.9 | about half-maximal activity at pH 5.6 and 7.9 | Malus sylvestris |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Malus sylvestris | - |
6.2 | 5.4 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
