Any feedback?
Literature summary for 2.3.1.99 extracted from
- Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves (1987), J. Plant Physiol., 131, 61-73.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | activation | Secale cereale |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
feruloyl-CoA | - |
Secale cereale | |
| 4.3 | - |
quinate | - |
Secale cereale | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Secale cereale | - |
rye, var. Kustro | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Secale cereale |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | primary | Secale cereale | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0042 | - |
- |
Secale cereale |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, at least 6 months | Secale cereale |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + quinate | at 39% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + 5-O-(4-coumaroyl)quinate | - |
? | |
| caffeoyl-CoA + quinate | condensation at 10% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + 5-O-caffeoylquinate | i.e. chlorogenic acid | ? | |
| feruloyl-CoA + quinate | best substrate | Secale cereale | CoA + O-feruloylquinate | - |
? | |
| p-coumaroyl-CoA + shikimate | - |
Secale cereale | CoA + 5-O-(p-coumaroyl)shikimate | - |
? | |
| sinapoyl-CoA + quinate | at 21% the rate of the reaction with feruloyl-CoA | Secale cereale | CoA + 5-O-sinapoylquinate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
denaturation after 5 min | Secale cereale |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
100 mM potassium phosphate buffer | Secale cereale |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 8 | about 80% of maximal activity at pH 6.9 and 8.0 | Secale cereale |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
