Reference to 2.3.1.98; Id = 719900 - BRENDA Enzyme Database

gene SlCGT, functional expression in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain GV2260 transfection method

Solanum lycopersicum

D328A

site-directed mutagenesis, the mutant shows 40% reduced activity

Solanum lycopersicum

S27A

site-directed mutagenesis, the mutant shows 13% reduced activity

Solanum lycopersicum

PMSF

treatment of purified SlCGT with 1, 10, and 50 mM PMSF leads to strong decreases in caffeoyl transfer activities to 60%, 40%, and 0% activity, respectively

Solanum lycopersicum

apoplast

apoplastic space of tomato leaves

Solanum lycopersicum

48046

-

additional information

SiCGT contains a putative targeting signal of 24 amino acids indicating an entry of the SlCGT into the secretory pathway

Solanum lycopersicum

-

39950

-

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

40000

-

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

42580

-

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

chlorogenate + glucarate

Solanum lycopersicum

the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor

quinate + 2-O-caffeoylglucarate

-

?

Solanum lycopersicum

E7AIM3

cv. Moneymaker, gene SlCGT, single-copy gene

-

native SlCGT 585fold from seedlings by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography

Solanum lycopersicum

flower

-

Solanum lycopersicum

-

fruit

at different stages of ripening

Solanum lycopersicum

-

leaf

apoplastic space of tomato leaves

Solanum lycopersicum

-

additional information

tissue expression pattern, overview. Transcripts of SlCGT, enzyme activity, and caffeoylglucarate accumulate preferentially in tomato leaves and flowers

Solanum lycopersicum

-

root

-

Solanum lycopersicum

-

seedling

-

Solanum lycopersicum

-

stem

-

Solanum lycopersicum

-

1.45

-

purified native enzyme, pH 6.0, 30Â°C

Solanum lycopersicum

chlorogenate + glucarate

-

719900

Solanum lycopersicum

quinate + 2-O-caffeoylglucarate

-

?

chlorogenate + glucarate

the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor

719900

Solanum lycopersicum

quinate + 2-O-caffeoylglucarate

-

?

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

More

peptide sequences are obtained by trypsin digestion and spectrometric sequencing, structure-function analysis, overview

Solanum lycopersicum

CGT

-

Solanum lycopersicum

chlorogenate:glucarate caffeoyltransferase

-

Solanum lycopersicum

30

-

assay at

Solanum lycopersicum

6

-

assay at

Solanum lycopersicum

sequence calculation, mature CGT without signal sequence

-

5.01

Solanum lycopersicum

sequence calculation, CGT with signal sequence

-

5.25

gene SlCGT, functional expression in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain GV2260 transfection method

Solanum lycopersicum

D328A

site-directed mutagenesis, the mutant shows 40% reduced activity

Solanum lycopersicum

S27A

site-directed mutagenesis, the mutant shows 13% reduced activity

Solanum lycopersicum

PMSF

treatment of purified SlCGT with 1, 10, and 50 mM PMSF leads to strong decreases in caffeoyl transfer activities to 60%, 40%, and 0% activity, respectively

Solanum lycopersicum

apoplast

apoplastic space of tomato leaves

Solanum lycopersicum

48046

-

additional information

SiCGT contains a putative targeting signal of 24 amino acids indicating an entry of the SlCGT into the secretory pathway

Solanum lycopersicum

-

39950

-

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

40000

-

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

42580

-

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

chlorogenate + glucarate

Solanum lycopersicum

the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor

quinate + 2-O-caffeoylglucarate

-

?

native SlCGT 585fold from seedlings by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography

Solanum lycopersicum

flower

-

Solanum lycopersicum

-

fruit

at different stages of ripening

Solanum lycopersicum

-

leaf

apoplastic space of tomato leaves

Solanum lycopersicum

-

additional information

tissue expression pattern, overview. Transcripts of SlCGT, enzyme activity, and caffeoylglucarate accumulate preferentially in tomato leaves and flowers

Solanum lycopersicum

-

root

-

Solanum lycopersicum

-

seedling

-

Solanum lycopersicum

-

stem

-

Solanum lycopersicum

-

1.45

-

purified native enzyme, pH 6.0, 30Â°C

Solanum lycopersicum

chlorogenate + glucarate

-

719900

Solanum lycopersicum

quinate + 2-O-caffeoylglucarate

-

?

chlorogenate + glucarate

the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor

719900

Solanum lycopersicum

quinate + 2-O-caffeoylglucarate

-

?

x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation

Solanum lycopersicum

More

peptide sequences are obtained by trypsin digestion and spectrometric sequencing, structure-function analysis, overview

Solanum lycopersicum

30

-

assay at

Solanum lycopersicum

6

-

assay at

Solanum lycopersicum

sequence calculation, mature CGT without signal sequence

-

5.01

Solanum lycopersicum

sequence calculation, CGT with signal sequence

-

5.25

evolution

the sequence determines SlCGT as a GDSL lipase-like protein, representing it as a member of the SGNH protein superfamily. Lipases of this family employ a catalytic triad of Ser-Asp-His with Ser as nucleophile of the GDSL motif. The catalytic triad of the GDSL lipase is not essential for SlCGT enzymatic activity. SlCGT is therefore an example of a GDSL lipase-like protein that has lost hydrolytic activity and has acquired a completely new function in plant metabolism, functioning in secondary metabolism as acyltransferase in synthesis of hydroxycinnamate esters by employing amino acid residues different from the lipase catalytic triad

Solanum lycopersicum

additional information

the deduced SlCGT amino acid sequence matches the conservation of the serine catalytic motif GXSXXDXG within the first consensus block near the N-terminus. A serine moiety of the GDSL protein may be part of the catalytic center in SlCGT

Solanum lycopersicum

evolution

the sequence determines SlCGT as a GDSL lipase-like protein, representing it as a member of the SGNH protein superfamily. Lipases of this family employ a catalytic triad of Ser-Asp-His with Ser as nucleophile of the GDSL motif. The catalytic triad of the GDSL lipase is not essential for SlCGT enzymatic activity. SlCGT is therefore an example of a GDSL lipase-like protein that has lost hydrolytic activity and has acquired a completely new function in plant metabolism, functioning in secondary metabolism as acyltransferase in synthesis of hydroxycinnamate esters by employing amino acid residues different from the lipase catalytic triad

Solanum lycopersicum

additional information

the deduced SlCGT amino acid sequence matches the conservation of the serine catalytic motif GXSXXDXG within the first consensus block near the N-terminus. A serine moiety of the GDSL protein may be part of the catalytic center in SlCGT

Solanum lycopersicum

719900

Teutschbein

Identification and localizatio ...

Solanum lycopersicum

J. Biol. Chem.

285

38374-38381

2010

-

1

-

2

-

1

-

2

-

3

1

-

6

-

1

-

8

1

-

2

1

-

1

-

2

-

1

-

2

-

1

-

2

-

3

1

-

1

-

8

1

-

2

1

-

1

-

2

-

2

-

487780

Strack

Properties and activity change ...

Solanum lycopersicum

Plant Physiol.

92

41-47

1990

-

2

1

3

-

3

1

2

-

3

-

1

-

1

5

-

1

-

1

-

2

-

1

-

3

-

3

1

2

-

1

-

1

5

-

1

-

1

-

Identification and localization of a lipase-like acyltransferase in phenylpropanoid metabolism of tomato (Solanum lycopersicum)

Data extracted from this reference: