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show all sequences of 2.3.1.98

Identification and localization of a lipase-like acyltransferase in phenylpropanoid metabolism of tomato (Solanum lycopersicum)

Teutschbein, J.; Gross, W.; Nimtz, M.; Milkowski, C.; Hause, B.; Strack, D.; J. Biol. Chem. 285, 38374-38381 (2010) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene SlCGT, functional expression in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain GV2260 transfection method
Solanum lycopersicum
Engineering
Protein Variants
Commentary
Organism
D328A
site-directed mutagenesis, the mutant shows 40% reduced activity
Solanum lycopersicum
S27A
site-directed mutagenesis, the mutant shows 13% reduced activity
Solanum lycopersicum
Inhibitors
Inhibitors
Commentary
Organism
Structure
PMSF
treatment of purified SlCGT with 1, 10, and 50 mM PMSF leads to strong decreases in caffeoyl transfer activities to 60%, 40%, and 0% activity, respectively
Solanum lycopersicum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
apoplast
apoplastic space of tomato leaves
Solanum lycopersicum
48046
-
additional information
SiCGT contains a putative targeting signal of 24 amino acids indicating an entry of the SlCGT into the secretory pathway
Solanum lycopersicum
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39950
-
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
40000
-
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
42580
-
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
chlorogenate + glucarate
Solanum lycopersicum
the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor
quinate + 2-O-caffeoylglucarate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Solanum lycopersicum
E7AIM3
cv. Moneymaker, gene SlCGT, single-copy gene
-
Purification (Commentary)
Purification (Commentary)
Organism
native SlCGT 585fold from seedlings by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography
Solanum lycopersicum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Solanum lycopersicum
-
fruit
at different stages of ripening
Solanum lycopersicum
-
leaf
apoplastic space of tomato leaves
Solanum lycopersicum
-
additional information
tissue expression pattern, overview. Transcripts of SlCGT, enzyme activity, and caffeoylglucarate accumulate preferentially in tomato leaves and flowers
Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
seedling
-
Solanum lycopersicum
-
stem
-
Solanum lycopersicum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.45
-
purified native enzyme, pH 6.0, 30°C
Solanum lycopersicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
chlorogenate + glucarate
-
719900
Solanum lycopersicum
quinate + 2-O-caffeoylglucarate
-
-
-
?
chlorogenate + glucarate
the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor
719900
Solanum lycopersicum
quinate + 2-O-caffeoylglucarate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
More
peptide sequences are obtained by trypsin digestion and spectrometric sequencing, structure-function analysis, overview
Solanum lycopersicum
Synonyms
Synonyms
Commentary
Organism
CGT
-
Solanum lycopersicum
chlorogenate:glucarate caffeoyltransferase
-
Solanum lycopersicum
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Solanum lycopersicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Solanum lycopersicum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Solanum lycopersicum
sequence calculation, mature CGT without signal sequence
-
5.01
Solanum lycopersicum
sequence calculation, CGT with signal sequence
-
5.25
Cloned(Commentary) (protein specific)
Commentary
Organism
gene SlCGT, functional expression in Nicotiana benthamiana leaves using Agrobacterium tumefaciens strain GV2260 transfection method
Solanum lycopersicum
Engineering (protein specific)
Protein Variants
Commentary
Organism
D328A
site-directed mutagenesis, the mutant shows 40% reduced activity
Solanum lycopersicum
S27A
site-directed mutagenesis, the mutant shows 13% reduced activity
Solanum lycopersicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
PMSF
treatment of purified SlCGT with 1, 10, and 50 mM PMSF leads to strong decreases in caffeoyl transfer activities to 60%, 40%, and 0% activity, respectively
Solanum lycopersicum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
apoplast
apoplastic space of tomato leaves
Solanum lycopersicum
48046
-
additional information
SiCGT contains a putative targeting signal of 24 amino acids indicating an entry of the SlCGT into the secretory pathway
Solanum lycopersicum
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39950
-
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
40000
-
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
42580
-
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
chlorogenate + glucarate
Solanum lycopersicum
the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor
quinate + 2-O-caffeoylglucarate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native SlCGT 585fold from seedlings by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography
Solanum lycopersicum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Solanum lycopersicum
-
fruit
at different stages of ripening
Solanum lycopersicum
-
leaf
apoplastic space of tomato leaves
Solanum lycopersicum
-
additional information
tissue expression pattern, overview. Transcripts of SlCGT, enzyme activity, and caffeoylglucarate accumulate preferentially in tomato leaves and flowers
Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
seedling
-
Solanum lycopersicum
-
stem
-
Solanum lycopersicum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.45
-
purified native enzyme, pH 6.0, 30°C
Solanum lycopersicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
chlorogenate + glucarate
-
719900
Solanum lycopersicum
quinate + 2-O-caffeoylglucarate
-
-
-
?
chlorogenate + glucarate
the enzyme catalyzes the formation of caffeoylglucarate and caffeoylgalactarate using chlorogenate, i.e. 5-O-caffeoylquinate, as acyl donor
719900
Solanum lycopersicum
quinate + 2-O-caffeoylglucarate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40000, about, SDS-PAGE, x * 39950, mature CGT without signal sequence, sequence calculation, x * 42580, CGT with signal sequence, sequence calculation
Solanum lycopersicum
More
peptide sequences are obtained by trypsin digestion and spectrometric sequencing, structure-function analysis, overview
Solanum lycopersicum
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Solanum lycopersicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Solanum lycopersicum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Solanum lycopersicum
sequence calculation, mature CGT without signal sequence
-
5.01
Solanum lycopersicum
sequence calculation, CGT with signal sequence
-
5.25
General Information
General Information
Commentary
Organism
evolution
the sequence determines SlCGT as a GDSL lipase-like protein, representing it as a member of the SGNH protein superfamily. Lipases of this family employ a catalytic triad of Ser-Asp-His with Ser as nucleophile of the GDSL motif. The catalytic triad of the GDSL lipase is not essential for SlCGT enzymatic activity. SlCGT is therefore an example of a GDSL lipase-like protein that has lost hydrolytic activity and has acquired a completely new function in plant metabolism, functioning in secondary metabolism as acyltransferase in synthesis of hydroxycinnamate esters by employing amino acid residues different from the lipase catalytic triad
Solanum lycopersicum
additional information
the deduced SlCGT amino acid sequence matches the conservation of the serine catalytic motif GXSXXDXG within the first consensus block near the N-terminus. A serine moiety of the GDSL protein may be part of the catalytic center in SlCGT
Solanum lycopersicum
General Information (protein specific)
General Information
Commentary
Organism
evolution
the sequence determines SlCGT as a GDSL lipase-like protein, representing it as a member of the SGNH protein superfamily. Lipases of this family employ a catalytic triad of Ser-Asp-His with Ser as nucleophile of the GDSL motif. The catalytic triad of the GDSL lipase is not essential for SlCGT enzymatic activity. SlCGT is therefore an example of a GDSL lipase-like protein that has lost hydrolytic activity and has acquired a completely new function in plant metabolism, functioning in secondary metabolism as acyltransferase in synthesis of hydroxycinnamate esters by employing amino acid residues different from the lipase catalytic triad
Solanum lycopersicum
additional information
the deduced SlCGT amino acid sequence matches the conservation of the serine catalytic motif GXSXXDXG within the first consensus block near the N-terminus. A serine moiety of the GDSL protein may be part of the catalytic center in SlCGT
Solanum lycopersicum
Other publictions for EC 2.3.1.98
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719900
Teutschbein
Identification and localizatio ...
Solanum lycopersicum
J. Biol. Chem.
285
38374-38381
2010
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1
-
2
-
1
-
2
-
3
1
-
6
-
-
1
-
-
8
1
-
2
2
2
1
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
2
-
-
1
-
-
2
-
3
1
-
-
-
1
-
8
1
-
2
2
1
-
-
-
1
-
-
2
-
2
2
-
-
-
487780
Strack
Properties and activity change ...
Solanum lycopersicum
Plant Physiol.
92
41-47
1990
-
-
-
-
-
2
1
3
-
3
1
2
-
3
-
-
1
-
-
1
1
1
5
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
3
-
3
1
2
-
-
-
1
-
1
1
1
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-