BRENDA - Enzyme Database
show all sequences of 2.3.1.98

Properties and activity changes of chlorogenic acid:glucaric acid caffeoyltransferase from tomato (Lycopersicon esculentum)

Strack, D.; Gross, W.; Plant Physiol. 92, 41-47 (1990) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

General Stability
General Stability
Organism
freezing and thawing causes 20-30% loss of activity
Solanum lycopersicum
purification has to be carried out at 4°C
Solanum lycopersicum
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
p-chloromercuribenzoate is no inhibitor
Solanum lycopersicum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
glucaric acid
-
Solanum lycopersicum
1.7
-
galactaric acid
-
Solanum lycopersicum
20
-
chlorogenic acid
-
Solanum lycopersicum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
150% of activity compared to the activity without addition of divalent ions
Solanum lycopersicum
Mg2+
125% of activity compared to the activity without addition of divalent ions
Solanum lycopersicum
additional information
EDTA has no effect
Solanum lycopersicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE, gel filtration
Solanum lycopersicum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-O-caffeoylquinic acid + galactaric acid
Solanum lycopersicum
-
quinate + caffeoylgalactaric acid
-
-
?
5-O-caffeoylquinic acid + glucaric acid
Solanum lycopersicum
-
quinate + caffeoylglucaric acid
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Solanum lycopersicum
-
tomato
-
Purification (Commentary)
Purification (Commentary)
Organism
2400fold
Solanum lycopersicum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Solanum lycopersicum
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Solanum lycopersicum
Storage Stability
Storage Stability
Organism
-20°C, several months, no apparent loss of activity with crude extract or purified enzyme
Solanum lycopersicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5-O-caffeoylquinic acid + galactaric acid
-
487780
Solanum lycopersicum
quinate + caffeoylgalactaric acid
-
-
-
?
5-O-caffeoylquinic acid + galactaric acid
-
487780
Solanum lycopersicum
quinate + caffeoylgalactaric acid
-
487780
Solanum lycopersicum
?
5-O-caffeoylquinic acid + glucaric acid
-
487780
Solanum lycopersicum
quinate + caffeoylglucaric acid
-
-
-
?
5-O-caffeoylquinic acid + glucaric acid
i.e. chlorogenic acid
487780
Solanum lycopersicum
quinate + caffeoylglucaric acid
-
487780
Solanum lycopersicum
?
chlorogenic acid + glucarate
-
487780
Solanum lycopersicum
quinate + 2-O-caffeoylglucarate
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
-
Solanum lycopersicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.7
-
-
Solanum lycopersicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.2
6.2
50% of maximal activity at pH 4.2 and pH 6.2
Solanum lycopersicum
General Stability (protein specific)
General Stability
Organism
freezing and thawing causes 20-30% loss of activity
Solanum lycopersicum
purification has to be carried out at 4°C
Solanum lycopersicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
p-chloromercuribenzoate is no inhibitor
Solanum lycopersicum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.4
-
glucaric acid
-
Solanum lycopersicum
1.7
-
galactaric acid
-
Solanum lycopersicum
20
-
chlorogenic acid
-
Solanum lycopersicum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
150% of activity compared to the activity without addition of divalent ions
Solanum lycopersicum
Mg2+
125% of activity compared to the activity without addition of divalent ions
Solanum lycopersicum
additional information
EDTA has no effect
Solanum lycopersicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
SDS-PAGE, gel filtration
Solanum lycopersicum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5-O-caffeoylquinic acid + galactaric acid
Solanum lycopersicum
-
quinate + caffeoylgalactaric acid
-
-
?
5-O-caffeoylquinic acid + glucaric acid
Solanum lycopersicum
-
quinate + caffeoylglucaric acid
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
2400fold
Solanum lycopersicum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Solanum lycopersicum
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Solanum lycopersicum
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, several months, no apparent loss of activity with crude extract or purified enzyme
Solanum lycopersicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5-O-caffeoylquinic acid + galactaric acid
-
487780
Solanum lycopersicum
quinate + caffeoylgalactaric acid
-
-
-
?
5-O-caffeoylquinic acid + galactaric acid
-
487780
Solanum lycopersicum
quinate + caffeoylgalactaric acid
-
487780
Solanum lycopersicum
?
5-O-caffeoylquinic acid + glucaric acid
-
487780
Solanum lycopersicum
quinate + caffeoylglucaric acid
-
-
-
?
5-O-caffeoylquinic acid + glucaric acid
i.e. chlorogenic acid
487780
Solanum lycopersicum
quinate + caffeoylglucaric acid
-
487780
Solanum lycopersicum
?
chlorogenic acid + glucarate
-
487780
Solanum lycopersicum
quinate + 2-O-caffeoylglucarate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
38
-
-
Solanum lycopersicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.7
-
-
Solanum lycopersicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.2
6.2
50% of maximal activity at pH 4.2 and pH 6.2
Solanum lycopersicum
Other publictions for EC 2.3.1.98
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719900
Teutschbein
Identification and localizatio ...
Solanum lycopersicum
J. Biol. Chem.
285
38374-38381
2010
-
-
1
-
2
-
1
-
2
-
3
1
-
6
-
-
1
-
-
8
1
-
2
2
2
1
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
2
-
-
1
-
-
2
-
3
1
-
-
-
1
-
8
1
-
2
2
1
-
-
-
1
-
-
2
-
2
2
-
-
-
487780
Strack
Properties and activity change ...
Solanum lycopersicum
Plant Physiol.
92
41-47
1990
-
-
-
-
-
2
1
3
-
3
1
2
-
3
-
-
1
-
-
1
1
1
5
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
3
-
3
1
2
-
-
-
1
-
1
1
1
5
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-