Application | Comment | Organism |
---|---|---|
synthesis | recombinant expression of N-myristoylated proteins in Escherichia coli can be achieved by co-expressing N-myristoyltransferase and supplementing the growth medium with myristic acid. Undesired incorporation of the 12-carbon fatty acid lauric acid can also occur, leading to heterogeneous samples. A strategy for obtaining lauryl-free samples of myristoylated proteins in both rich and minimal media | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lauroyl-CoA + brain acid soluble protein 1 | - |
Homo sapiens | N-lauroyl-brain acid soluble protein 1 + CoA | N-laurylation of recombinant proteins in Escherichia coli may occur in minimal media | ? | |
lauroyl-CoA + c-Src | N-terminal region of c-Src, residues 1-185 | Homo sapiens | N-lauroyl-c-Src + CoA | N-laurylation of recombinant proteins in Escherichia coli may occur in minimal media | ? | |
myristoyl-CoA + brain acid soluble protein 1 | - |
Homo sapiens | N-myristoyl-brain acid soluble protein 1 + CoA | - |
? | |
myristoyl-CoA + c-Src | N-terminal region of c-Src, residues 1-185 | Homo sapiens | N-myristoyl-c-Src + CoA | - |
? |