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Literature summary for 2.3.1.97 extracted from

  • Goncalves, V.; Brannigan, J.A.; Thinon, E.; Olaleye, T.O.; Serwa, R.; Lanzarone, S.; Wilkinson, A.J.; Tate, E.W.; Leatherbarrow, R.J.
    A fluorescence-based assay for N-myristoyltransferase activity (2012), Anal. Biochem., 421, 342-344.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.97

Inhibitors

Inhibitors Comment Organism Structure
2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
-
 Homo sapiens
N-(11-aminoundecanoyl)-L-seryl-N-(2-cyclohexylethyl)-L-lysinamide
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00276
-
 peptide Hs pp60src(2-9) pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens
0.00277
-
 peptide Hs pp60src(2-9) pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens
0.00724
-
 myristoyl-CoA pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens
0.00824
-
 myristoyl-CoA pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
tetradecanoyl-CoA + glycylpeptide Homo sapiens posttranslational transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins CoA + N-tetradecanoylglycylpeptide
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 isozymes NMT1 and NMT2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information development of a robust sensitive fluorescence-based assay method to study the enzymatic activity of NMT1 and NMT2 based on detection of CoA by 7-diethylamino-3-(4-maleimido-phenyl)-4-methylcoumarin, overview Homo sapiens ?
-
 ?
myristoyl-CoA + peptide Hs pp60src(2-9)
-
 Homo sapiens CoA + N-myristoyl-peptideHs pp60src(2-9)
-
 ?
tetradecanoyl-CoA + glycylpeptide posttranslational transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins Homo sapiens CoA + N-tetradecanoylglycylpeptide
-
 ?
tetradecanoyl-CoA + glycylpeptide transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins Homo sapiens CoA + N-tetradecanoylglycylpeptide
-
 ?

Synonyms

Synonyms Comment Organism
myristoyl-CoA:protein N-myristoyltransferase
-
 Homo sapiens
myristoyl-coenzyme A:protein N-myristoyltransferase
-
 Homo sapiens
N-myristoyltransferase
-
 Homo sapiens
NMT
-
 Homo sapiens
NMT1
-
 Homo sapiens
NMT2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9 8 assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000137
-
 pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
0.0000144
-
 pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens 2,6-dichloro-4-[2-(piperazin-1-yl)pyridin-4-yl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)benzenesulfonamide
0.000349
-
 pH 7.9-8.0, 25°C, isozyme NMT1 Homo sapiens N-(11-aminoundecanoyl)-L-seryl-N-(2-cyclohexylethyl)-L-lysinamide
0.000513
-
 pH 7.9-8.0, 25°C, isozyme NMT2 Homo sapiens N-(11-aminoundecanoyl)-L-seryl-N-(2-cyclohexylethyl)-L-lysinamide

General Information

General Information Comment Organism
physiological function myristoyl-CoA:protein N-myristoyltransferase is a ubiquitous enzyme in eukaryotes that catalyzes the co- and posttranslational transfer of a C14 saturated fatty acid (myristic acid) from myristoyl-CoA to the N-terminal glycine residue of target proteins Homo sapiens