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Literature summary for 2.3.1.97 extracted from

  • Raju, R.V.S.; Datla, R.S.S.; Warrington, R.C.; Sharma, R.K.
    Effects of L-histidine and its structural analogs on human N-myristoyltransferase activity and importance of EEVEH amino acid sequence for enzyme activity (1998), Biochemistry, 37, 14928-14936.
    View publication on PubMed
Search for more literature for 2.3.1.97

Activating Compound

Activating Compound Comment Organism Structure
L-histidine activation in a concentration dependent manner Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants from Escherichia coli DH5alpha Homo sapiens

Protein Variants

Protein Variants Comment Organism
E289G site-directed mutagenesis, 37.8% remaining activity Homo sapiens
E289G/E290G site-directed mutagenesis, no remaining activity Homo sapiens
E289G/E290G/E292G site-directed mutagenesis, no remaining activity Homo sapiens
E289G/E292G site-directed mutagenesis, 7% remaining activity Homo sapiens
E290G site-directed mutagenesis, no remaining activity Homo sapiens
E292G site-directed mutagenesis, 77.6% remaining activity Homo sapiens
E292H site-directed mutagenesis, 76% remaining activity Homo sapiens
H293G site-directed mutagenesis, no remaining activity Homo sapiens
H293N site-directed mutagenesis, no remaining activity Homo sapiens
V291G site-directed mutagenesis, no remaining activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
histamine noncompetitive Homo sapiens
L-histidinol noncompetitive, reversible by L-histidine Homo sapiens
additional information inhibition mechanism Homo sapiens
additional information
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.195
-
 L-histidine
-
 Homo sapiens
0.2
-
 cAMP-dependent protein kinase-derived peptide
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] Glu290, Val-291 and His293 within conserved region EEVEH, are essential for catalysis Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myristoyl-CoA + glycylpeptide
-
 Homo sapiens N-myristoylglycylpeptide + CoA
-
 ?
myristoyl-CoA + glycylpeptide
-
 Rattus norvegicus N-myristoylglycylpeptide + CoA
-
 ?

Synonyms

Synonyms Comment Organism
NMT
-
 Homo sapiens
NMT
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens