Cloned (Comment) | Organism |
---|---|
construction of expression plasmid encoding the enzyme, lacking the first 8 amino acid residues, fused to a enterokinase cleavage site and a polyhistidine tag, expression in Escherichia coli DH5alpha, cDNA from spleen | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
p60src-derived peptide | recombinant enzyme | Bos taurus | |
0.2 | - |
cAMP-dependent protein kinase-derived peptide | recombinant enzyme | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
spleen enzyme, recombinant from Escherichia coli | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.048 | - |
purified recombinant protein | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myristoyl-CoA + cAMP-dependent protein kinase-derived peptide | - |
Bos taurus | N-myristoylated cAMP-dependent protein kinase-derived peptide + CoA | - |
? | |
myristoyl-CoA + glycylpeptide | - |
Bos taurus | N-myristoylglycylpeptide + CoA | - |
? | |
myristoyl-CoA + p60src-derived peptide | - |
Bos taurus | N-myristoylated p60src-derived peptide + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, tag cleaved off by enterokinase, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
NMT | - |
Bos taurus |