Literature summary for 2.3.1.97 extracted from

Zhang, L.; Jackson-Machelski, E.; Gordon, J.I.
Biochemical studies of Saccharomyces cerevisiae myristoyl-coenzyme A:protein N-myristoyltransferase mutants (1996), J. Biol. Chem., 271, 33131-33140.

Search for more literature for 2.3.1.97

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged wild-type and mutants in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
A202T	site-directed mutagenesis, alterations of both peptide and myristoyl binding sites, 3 to 6fold increased Ki for S-(2-oxo)-pentadecyl-CoA and 6 to 9fold increased Ki for SC-58272	Saccharomyces cerevisiae
C217R	site-directed mutagenesis, 3 to 6fold increase in Ki for inhibitor SC-58272, selective alteration of the enzymes peptide binding site	Saccharomyces cerevisiae
D417V	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae
F413S	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae
K389I	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae
L171S	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae
L408S	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae
L420S	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae
N102T	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae
N426I	site-directed mutagenesis, mutation of myristoyl-binding site	Saccharomyces cerevisiae
S328P	site-directed mutagenesis, highly reduced activity	Saccharomyces cerevisiae
V395D	site-directed mutagenesis, reduced activity, temperature-sensitive	Saccharomyces cerevisiae

Inhibitors

Inhibitors	Comment	Organism	Structure
S-(2-Oxo)-pentadecyl-CoA	competitive	Saccharomyces cerevisiae
SC-58272	peptidomimetic derived from the N-terminal sequence of the substrate ADP-ribosylation factor-2, i.e. Arf2p	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
myristoyl-CoA + glycylpeptide	Saccharomyces cerevisiae	enzyme is essential for vegetative growth	N-myristoylglycylpeptide + CoA	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	gene nmt1	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged recombinant wild-type and mutants from Escherichia coli	Saccharomyces cerevisiae

Reaction

Reaction	Comment	Organism	Reaction ID
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	ordered bi bi mechanism	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	peptide binding site	Saccharomyces cerevisiae	a
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]	myristoyl-CoA binding site	Saccharomyces cerevisiae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myristoyl-CoA + glycylpeptide	specific for Gly at N-terminus	Saccharomyces cerevisiae	N-myristoylglycylpeptide + CoA	-	?
myristoyl-CoA + glycylpeptide	enzyme is essential for vegetative growth	Saccharomyces cerevisiae	N-myristoylglycylpeptide + CoA	-	?

Synonyms

Synonyms	Comment	Organism
Nmt1p	-	Saccharomyces cerevisiae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	Ki of several mutants for S-(2-oxo)-pentadecyl-CoA at 24 and 37°C	Saccharomyces cerevisiae
0.000005	-	S-(2-Oxo)-pentadecyl-CoA	wild-type, 37°C	Saccharomyces cerevisiae
0.000009	-	SC-58272	His-tagged wild-type, 37°C	Saccharomyces cerevisiae
0.000014	-	SC-58272	wild-type, 37°C	Saccharomyces cerevisiae
0.000055	-	SC-58272	His-tagged mutant C217R, 37°C	Saccharomyces cerevisiae
0.000082	-	SC-58272	His-tagged mutant A202T, 37°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)