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Literature summary for 2.3.1.94 extracted from

  • Robbins, T.; Kapilivsky, J.; Cane, D.E.; Khosla, C.
    Roles of conserved active site residues in the ketosynthase domain of an assembly line polyketide synthase (2016), Biochemistry, 55, 4476-4484 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.94

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharopolyspora erythraea

Protein Variants

Protein Variants Comment Organism
C211A mutation at the ketosynthase domain active site, exhibits no turnover activity, but is still a competent methylmalonyl-ACP decarboxylase Saccharopolyspora erythraea
H346A mutation at the ketosynthase domain, mutant exhibits reduced rates of both chain translocation and chain elongation, with a greater effect on the latter half-reaction Saccharopolyspora erythraea
H384A mutation at the ketosynthase domain, residue contributes to methylmalonyl-ACP decarboxylation Saccharopolyspora erythraea
H457A activity similar to wild-type Saccharopolyspora erythraea
K379A mutation at the ketosynthase domain, residue promotes C-C bond formation Saccharopolyspora erythraea
N381A increase in activity Saccharopolyspora erythraea
N455L activity similar to wild-type Saccharopolyspora erythraea
Q386E about 25% of wild-type activity Saccharopolyspora erythraea
S315A mutation at the ketosynthase domain, residue plays a role in coupling decarboxylation to C-C bond formation Saccharopolyspora erythraea
T209A increase in activity Saccharopolyspora erythraea

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea Q03131 and Q03132 and Q03133 Q03131 i.e. modules 1 and 2, Q03132 i.e. modules 3 and 4, Q03133 i.e. modules 5 and 6
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propanoyl-CoA + (2S)-methylmalonyl-CoA + NADPH
-
 Saccharopolyspora erythraea (3R,4S,5R,6R)-6-ethyl-4-hydroxy-3,5-dimethyloxan-2-one + ?
-
 ?

Synonyms

Synonyms Comment Organism
eryA
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 Saccharopolyspora erythraea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0067
-
 propanoyl-CoA mutant H384A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
0.028
-
 propanoyl-CoA mutant Q386E, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
0.032
-
 propanoyl-CoA mutant H457A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
0.038
-
 propanoyl-CoA mutant N381A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
0.038
-
 propanoyl-CoA mutant N455L, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
0.04
-
 propanoyl-CoA mutant S315A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
0.042
-
 propanoyl-CoA wild-type, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
0.048
-
 propanoyl-CoA mutant T209A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea