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Literature summary for 2.3.1.94 extracted from

  • Kumpfmueller, J.; Methling, K.; Fang, L.; Pfeifer, B.A.; Lalk, M.; Schweder, T.
    Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis (2016), Appl. Microbiol. Biotechnol., 100, 1209-1220.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the deoxyerythronolide B synthase genes are functionally expressed in Bacillus subtilis when the native eryAI–III operon is separated into three individual expression cassettes with optimized ribosomal binding sites. A synthesis of 6-deoxyerythronolide B can be detected by using the acetoininducible acoA promoter and a fed-batch simulating EnBase-cultivation strategy. Bacillus subtilis is capable of the secretion of 6-deoxyerythronolide B into the medium. The deletion of the prpBD operon of Bacillus subtilis, responsible for propionyl-CoA utilization, results in a significant increase of the 6-deoxyerythronolide B product yield when exogenous propionate is provided Saccharopolyspora erythraea

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Saccharopolyspora erythraea

Organism

Organism UniProt Comment Textmining
Saccharopolyspora erythraea Q03131 and Q03132 and Q03133 Q03131, i.e. modules 1 and 2, Q03132, i.e. modules 3 and 4, Q03133, i.e. modules 5 and 6
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