Application | Comment | Organism |
---|---|---|
synthesis | the deoxyerythronolide B synthase genes are functionally expressed in Bacillus subtilis when the native eryAIIII operon is separated into three individual expression cassettes with optimized ribosomal binding sites. A synthesis of 6-deoxyerythronolide B can be detected by using the acetoininducible acoA promoter and a fed-batch simulating EnBase-cultivation strategy. Bacillus subtilis is capable of the secretion of 6-deoxyerythronolide B into the medium. The deletion of the prpBD operon of Bacillus subtilis, responsible for propionyl-CoA utilization, results in a significant increase of the 6-deoxyerythronolide B product yield when exogenous propionate is provided | Saccharopolyspora erythraea |
Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | Q03131 and Q03132 and Q03133 | Q03131, i.e. modules 1 and 2, Q03132, i.e. modules 3 and 4, Q03133, i.e. modules 5 and 6 | - |