Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by dithioerythritol and 2-mercaptoethanol | Raphanus sativus |
General Stability | Organism |
---|---|
Stable to repeated freeze-thawing | Raphanus sativus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-malate | above 0.14 M | Raphanus sativus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
1-O-sinapoyl-beta-D-glucose | - |
Raphanus sativus | |
54 | - |
L-malate | - |
Raphanus sativus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Raphanus sativus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Raphanus sativus | - |
red radish, var. sativus | - |
Purification (Comment) | Organism |
---|---|
partial | Raphanus sativus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | maximal activity in 10-14 days old cotyledons | Raphanus sativus | - |
Storage Stability | Organism |
---|---|
-20°C, 3 weeks | Raphanus sativus |
0°C, 3 weeks | Raphanus sativus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-O-sinapoyl-beta-D-glucose + L-malate | - |
Raphanus sativus | D-glucose + sinapoyl-L-malate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
14 h stable | Raphanus sativus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
various buffer systems | Raphanus sativus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 6.8 | about half-maximal activity at pH 5.7 and 6.8, various buffer systems | Raphanus sativus |