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Literature summary for 2.3.1.9 extracted from

  • Kim, S.; Jang, Y.S.; Ha, S.C.; Ahn, J.W.; Kim, E.J.; Lim, J.H.; Cho, C.; Ryu, Y.S.; Lee, S.K.; Lee, S.Y.; Kim, K.J.
    Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum (2015), Nature Commun., 6, 8410.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biofuel production the enzyme catalyses the condensation of two acetyl-coenzyme A molecules to form acetoacetyl-CoA in a dedicated pathway towards the biosynthesis of n-butanol, an important solvent and biofuel Clostridium acetobutylicum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834 cells Clostridium acetobutylicum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM phosphate-citrate pH 4.2, 10% (w/v) polyethylene glycol 3350, 200 mM sodium chloride Clostridium acetobutylicum

Protein Variants

Protein Variants Comment Organism
V77Q/N153Y/A286K the mutant, which is not able to form disulfide bonds, exhibits higher activity than the wild type enzyme Clostridium acetobutylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
x * 53000, SDS-PAGE Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 acetyl-CoA Clostridium acetobutylicum
-
CoA + acetoacetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum P45359
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and HiTrap Q ion exchange column chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 acetyl-CoA
-
Clostridium acetobutylicum CoA + acetoacetyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Clostridium acetobutylicum

Synonyms

Synonyms Comment Organism
ThL
-
Clostridium acetobutylicum