Literature summary for 2.3.1.9 extracted from

Janardan, N.; Harijan, R.K.; Kiema, T.R.; Wierenga, R.K.; Murthy, M.R.
Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis (2015), Acta Crystallogr. Sect. D, 71, 2479-2493.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment)	Organism
microbatch method, using either 20% (w/v) PEG 3350, 0.15 M calcium chloride dehydrate, or 45% (w/v) PEG 200, 0.1 M MES monohydrate pH 6.0, 0.07 M calcium chloride dehydrate or 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, 30% (w/v) PEG 8000, 5% (v/v) n-octyl-beta-D-glucoside	Mycolicibacterium smegmatis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 acetyl-CoA	Mycolicibacterium smegmatis	-	CoA + acetoacetyl-CoA	-	ir
additional information	Mycolicibacterium smegmatis	no activity is observed with acetyl-CoA	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-NTA bead chromatography and Superose 6 gel filtration	Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 acetyl-CoA	-	Mycolicibacterium smegmatis	CoA + acetoacetyl-CoA	-	ir
additional information	no activity is observed with acetyl-CoA	Mycolicibacterium smegmatis	?	-	?

Subunits

Subunits	Comment	Organism
homotetramer	x-ray crystallography	Mycolicibacterium smegmatis

Synonyms

Synonyms	Comment	Organism
3-ketoacyl-CoA (T1)-like thiolase	-	Mycolicibacterium smegmatis
MSM-13 thiolase	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)