Cloned (Comment) | Organism |
---|---|
AACT, DNA and amino acid sequence determination and analysis, sequence comparison with Arabidopsis thaliana, expression of the active His-tagged enzyme in Escherichia coli TOP10 cells | Helianthus annuus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glyoxysome | - |
Helianthus annuus | 9514 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, recombinant enzyme, SDS-PAGE | Helianthus annuus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | D2IH11 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AACT 88fold to homogeneity by nickel affinity chromatography from Escherichia coli TOP10 cells | Helianthus annuus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Helianthus annuus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.2 | - |
purified native enzyme | Helianthus annuus |
15.78 | - |
purified recombinant enzyme | Helianthus annuus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + CoA | coupled reaction assay in which the product of the thiolase reaction, acetyl-CoA, becomes the substrate for citrate synthase | Helianthus annuus | 2 acetyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, recombinant enzyme, SDS-PAGE | Helianthus annuus |
Synonyms | Comment | Organism |
---|---|---|
AACT | - |
Helianthus annuus |
acetoacetyl CoA thiolase | - |
Helianthus annuus |
thiolase II | - |
Helianthus annuus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Helianthus annuus |