Protein Variants | Comment | Organism |
---|---|---|
E252del | relative protein amount and enzyme activity of 30% and 25% respectively, in comparison to the wild-type at 37°C. 2fold Km-elevation for substrates coenzyme A and acetoacetyl-CoA compared to wild-type values. Vmax is comparable to wild-type value | Homo sapiens |
E252del | 25% of wild-type activity at 37°C, 40% of wild-type activity at 30°C. Mutant is unstable compared to the wild-type protein at 37°C. KM-value for acetoacetyl-CoA is 2fold higher than wild-type value. Km-value for CoA is 1.8fold lower than wild-type value | Homo sapiens |
E252del | no residual activity under any condition | Homo sapiens |
N158D | inactive mutant protein | Homo sapiens |
N158D | no residual activity under any condition | Homo sapiens |
N158S | inactive mutant protein | Homo sapiens |
N158S | no residual activity under any condition | Homo sapiens |
N282H | inactive mutant protein | Homo sapiens |
N282H | no residual activity under any condition | Homo sapiens |
N353K | cDNAs yield neither residual T2 protein nor enzyme activity | Homo sapiens |
N353K | no residual activity under any condition | Homo sapiens |
Q272X | no residual activity under any condition | Homo sapiens |
Q73P | cDNAs yield neither residual T2 protein nor enzyme activity | Homo sapiens |
Q73P | no residual activity under any condition | Homo sapiens |
R208Q | inactive mutant protein | Homo sapiens |
R208Q | no residual activity under any condition | Homo sapiens |
Y219H | inactive mutant protein | Homo sapiens |
Y219H | no residual activity under any condition | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | substrate inhibition beyond 0.0225 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
acetoacetyl-CoA | wild-type enzyme | Homo sapiens | |
0.005 | - |
acetoacetyl-CoA | E252del mutant enzyme | Homo sapiens | |
0.01 | - |
acetoacetyl-CoA | wild-type enzyme | Homo sapiens | |
0.01 | - |
acetoacetyl-CoA | mutant enzyme E252del | Homo sapiens | |
0.028 | - |
CoA | wild-type enzyme | Homo sapiens | |
0.05 | - |
CoA | mutant enzyme E252del | Homo sapiens | |
0.05 | - |
CoA | E252del mutant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + acetyl-CoA | - |
Homo sapiens | CoA + acetoacetyl-CoA | - |
? | |
CoA + acetoacetyl-CoA | - |
Homo sapiens | acetyl-CoA + acetyl-CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetoacetyl-CoA thiolase T2 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
wild-type T2 protein is stable even after chasing for 48 h at 37°C. The amount of E252del mutant protein at 48 h incubation with cycloheximide is estimated to be 50% of that observed at 0 h. E252del mutant T2 is unstable compared to the wild-type protein at 37°C | Homo sapiens |