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Literature summary for 2.3.1.9 extracted from

  • Antonenkov, V.D.; Croes, K.; Waelkens, E.; Van Veldhoven, P.P.; Mannaerts, G.P.
    Identification, purification and characterization of an acetoacetyl-CoA thiolase from rat liver peroxisomes (2000), Eur. J. Biochem., 267, 2981-2990.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 CoA peroxisomal thiolase, thiolysis Rattus norvegicus
0.009
-
 acetoacetyl-CoA peroxisomal thiolase, thiolysis Rattus norvegicus
0.016
-
 acetoacetyl-CoA cytosolic thiolase, thiolysis Rattus norvegicus
0.02
-
 CoA cytosolic thiolase, thiolysis Rattus norvegicus
0.035
-
 acetoacetyl-CoA peroxisomal thiolase, synthesis Rattus norvegicus
0.08
-
 acetoacetyl-CoA cytosolic thiolase, synthesis Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
 Rattus norvegicus 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 4 * 42000, peroxisomal thiolase, SDS-PAGE Rattus norvegicus
160000
-
 peroxisomal thiolase, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Rattus norvegicus peroxisomal thiolase most probably catalyzes the first reaction in peroxisomal cholesterol and dolichol synthesis acetyl-CoA + acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
peroxisomal thiolase, DEAE-cellulose, phosphocellulose column, Blue-Sepharose Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100 130 peroxisomal thiolase, thiolysis Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
 Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 r
CoA + acetoacetyl-CoA peroxisomal thiolase most probably catalyzes the first reaction in peroxisomal cholesterol and dolichol synthesis Rattus norvegicus acetyl-CoA + acetyl-CoA
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 42000, peroxisomal thiolase, SDS-PAGE Rattus norvegicus