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Literature summary for 2.3.1.9 extracted from

  • Kanayama, N.; Himeda, Y.; Atomi, H.; Ueda, M.; Tanaka, A.
    Expression of acetoacetyl-CoA thiolase isoenzyme genes of n-alkane-assimilating yeast, Candida tropicalis: isoenzymes in two intracellular compartments are derived from the same genes (1997), J. Biochem., 122, 616-621.
    View publication on PubMed
Search for more literature for 2.3.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expression of cytosolic and peroxisomal thiolase I in Saccharomyces cerevisiae Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 acetoacetyl-CoA recombinant cytosolic thiolase I, thiolysis Candida tropicalis
0.025
-
 acetoacetyl-CoA peroxisomal thiolase I, thiolysis Candida tropicalis
0.03
-
 CoA peroxisomal thiolase I, thiolysis Candida tropicalis
0.032
-
 CoA recombinant peroxisomal thiolase I, thiolysis Candida tropicalis
0.037
-
 CoA cytosolic thiolase I, thiolysis Candida tropicalis
0.042
-
 acetoacetyl-CoA cytosolic thiolase I, thiolysis Candida tropicalis
0.05
-
 CoA recombinant cytosolic thiolase I, thiolysis Candida tropicalis
0.057
-
 acetoacetyl-CoA recombinant peroxisomal thiolase I, thiolysis Candida tropicalis
0.69
-
 acetyl-CoA recombinant cytosolic thiolase I, thiolysis Candida tropicalis
0.74
-
 acetyl-CoA recombinant peroxisomal thiolase I, thiolysis Candida tropicalis
0.77
-
 acetyl-CoA cytosolic thiolase I, thiolysis Candida tropicalis
1.05
-
 acetyl-CoA peroxisomal thiolase I, thiolysis Candida tropicalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic thiolase I Candida tropicalis 5829
-
peroxisome peroxisomal thiolase I Candida tropicalis 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 6 * 41000, cytosolic and peroxisomal thiolase I, SDS-PAGE Candida tropicalis
240000
-
 peroxisomal thiolase I, gel filtration Candida tropicalis
250000
-
 cytosolic thiolase I, gel filtration Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Candida tropicalis
-
 acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA Rhizobium sp.
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
 ATCC 20336
-
Rhizobium sp.
-
 cicer, strain CC 1192
-

Purification (Commentary)

Purification (Comment) Organism
cytosolic thiolase I Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
59.8
-
-
 Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
 Candida tropicalis acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA
-
 Candida tropicalis acetyl-CoA + acetyl-CoA
-
 r
CoA + acetoacetyl-CoA
-
 Rhizobium sp. acetyl-CoA + acetyl-CoA
-
 ?
CoA + acetoacetyl-CoA
-
 Rhizobium sp. acetyl-CoA + acetyl-CoA
-
 r

Subunits

Subunits Comment Organism
hexamer 6 * 41000, cytosolic and peroxisomal thiolase I, SDS-PAGE Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
-
 Rhizobium sp.
8.1
-
 cytosolic and peroxisomal thiolase I, synthesis Candida tropicalis
8.3
-
 cytosolic and peroxisomal thiolase I Candida tropicalis