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Literature summary for 2.3.1.9 extracted from
- Acetoacetyl-CoA thiolase of Bradyrhizobium japonicum bacteroids: purification and properties (1987), Arch. Biochem. Biophys., 254, 272-281.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes | Bradyrhizobium japonicum |
| glycerol stabilizes | Bradyrhizobium japonicum |
| unstable in dilute solution, less than 0.5 mg/ml protein | Bradyrhizobium japonicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 0.5 mM, 96% inhibition, acetoacetyl-CoA partly protects | Bradyrhizobium japonicum |  |
| iodoacetate | - |
Bradyrhizobium japonicum | |
| Mg2+ | 60 mM, 45% inhibition of thiolysis, 69% inhibition of synthesis | Bradyrhizobium japonicum | |
| NADH | inhibition of thiolysis | Bradyrhizobium japonicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
acetoacetyl-CoA | - |
Bradyrhizobium japonicum | |
| 0.03 | - |
CoA | - |
Bradyrhizobium japonicum | |
| 0.104 | - |
acetyl-CoA | - |
Bradyrhizobium japonicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
4 * 44000, SDS-PAGE | Bradyrhizobium japonicum |
| 180000 | - |
gel filtration | Bradyrhizobium japonicum |
| 193000 | - |
native PAGE | Bradyrhizobium japonicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | Bradyrhizobium japonicum | - |
acetyl-CoA + acetyl-CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bradyrhizobium japonicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, hydroxylapatite, phosphocellulose | Bradyrhizobium japonicum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 acetyl-CoA = CoA + acetoacetyl-CoA | ping-pong mechanism | Bradyrhizobium japonicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bacteroid | - |
Bradyrhizobium japonicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 102.9 | - |
- |
Bradyrhizobium japonicum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in dilute solution, less than 0.5 mg/ml protein, 24 h, complete loss of activity | Bradyrhizobium japonicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | - |
Bradyrhizobium japonicum | acetyl-CoA + acetyl-CoA | - |
? | |
| CoA + acetoacetyl-CoA | specific for acetoacetyl-CoA, no activity with ketodecanoyl-CoA, dithiothreitol and 2-mercaptoethanol | Bradyrhizobium japonicum | acetyl-CoA + acetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 44000, SDS-PAGE | Bradyrhizobium japonicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
condensation | Bradyrhizobium japonicum |
| 7.8 | - |
thiolysis | Bradyrhizobium japonicum |
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