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Literature summary for 2.3.1.9 extracted from

  • Palmer, M.A.J.; Differding, E.; Gamboni, R.; Williams, S.F.; Peoples, O.P.; Walsh, C.T.; Sinskey, A.J.; Masamune, S.
    Biosynthetic thiolase from Zoogloea ramigera. Evidence for a mechanism involving Cys-378 as the active site base (1991), J. Biol. Chem., 266, 8369-8375.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
C378G mutation eliminates the ability of thiolase to catalyze proton abstraction from C2 of acetyl-CoA Zoogloea ramigera

Inhibitors

Inhibitors Comment Organism Structure
2,3-pentadienoyl-S-pantetheine 11-pivalate half-life for inactivation: 1.9 min Zoogloea ramigera
3-Pentenoyl-S-pantetheine 11-pivalate half-life for inactivation: 0.26 min Zoogloea ramigera
Acryl-S-pantetheine 11-pivalate
-
Zoogloea ramigera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Zoogloea ramigera
-
acetyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Zoogloea ramigera
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
?
CoA + acetoacetyl-CoA
-
Zoogloea ramigera acetyl-CoA + acetyl-CoA
-
r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.25
-
3-Pentenoyl-S-pantetheine 11-pivalate
-
Zoogloea ramigera
1.54
-
2,3-pentadienoyl-S-pantetheine 11-pivalate
-
Zoogloea ramigera