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Literature summary for 2.3.1.9 extracted from

  • Bloxham, D.P.; Chalkley, R.A.; Coghlin, S.J.; Salam, W.
    Synthesis of chloromethyl ketone derivatives of fatty acids. Their use as specific inhibitors of acetoacetyl-coenzyme A thiolase, cholesterol biosynthesis and fatty acid synthesis (1978), Biochem. J., 175, 999-1011.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
11-Chloro-10-oxoundecanoic acid acetyl-CoA or CoA protect Sus scrofa
11-chloro-10-oxoundecanoyl-CoA acetyl-CoA or CoA protect Sus scrofa
5-chloro-4-oxopentanoyl-CoA acetyl-CoA or CoA protect Sus scrofa
7-Chloro-6-oxoheptanoic acid acetyl-CoA or CoA protect Sus scrofa
7-chloro-6-oxoheptanoyl-CoA acetyl-CoA or CoA protect Sus scrofa
9-Chloro-8-oxononanoic acid acetyl-CoA or CoA protect Sus scrofa
9-chloro-8-oxononanoyl-CoA acetyl-CoA or CoA protect Sus scrofa
citraconic anhydride
-
Sus scrofa
dec-3-ynoic acid irreversible inhibition Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + acetoacetyl-CoA Sus scrofa
-
acetyl-CoA + acetyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, acetone, acid treatment, CM-Sephadex, Sepharose 6B Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.2
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + acetoacetyl-CoA
-
Sus scrofa acetyl-CoA + acetyl-CoA
-
?
CoA + acetoacetyl-CoA
-
Sus scrofa acetyl-CoA + acetyl-CoA
-
r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0014
-
9-chloro-8-oxononanoyl-CoA
-
Sus scrofa
0.002
-
7-chloro-6-oxoheptanoyl-CoA
-
Sus scrofa
0.0025
-
11-chloro-10-oxoundecanoyl-CoA
-
Sus scrofa
0.015
-
5-chloro-4-oxopentanoyl-CoA
-
Sus scrofa
0.028
-
11-Chloro-10-oxoundecanoic acid
-
Sus scrofa
0.49
-
9-Chloro-8-oxononanoic acid
-
Sus scrofa
10
-
dec-3-ynoic acid
-
Sus scrofa
11.4
-
7-Chloro-6-oxoheptanoic acid
-
Sus scrofa