BRENDA - Enzyme Database show
show all sequences of 2.3.1.9

Purification and properties of beta-ketothiolase from Zoogloea ramigera

Nishimura, T.; Saito, T.; Tomita, K.; Arch. Microbiol. 116, 21-27 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
0.4 mM, 87% inhibition
Zoogloea ramigera
iodoacetamide
0.4 mM, 36% inhibition
Zoogloea ramigera
N-ethylmaleimide
0.4 mM, 53% inhibition
Zoogloea ramigera
NADH
inhibition of thiolysis
Zoogloea ramigera
NADPH
-
Zoogloea ramigera
p-chloromercuribenzoate
0.4 mM, 95% inhibition
Zoogloea ramigera
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0085
-
CoA
-
Zoogloea ramigera
0.01
-
acetoacetyl-CoA
-
Zoogloea ramigera
0.33
-
acetyl-CoA
-
Zoogloea ramigera
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
4 * 44000, SDS-PAGE
Zoogloea ramigera
190000
-
gel filtration
Zoogloea ramigera
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA + acetoacetyl-CoA
Zoogloea ramigera
-
acetyl-CoA + acetyl-CoA
-
Zoogloea ramigera
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zoogloea ramigera
-
-
-
Purification (Commentary)
Commentary
Organism
protamine sulfate, DEAE-cellulose, phosphocellulose, Sephadex G-200
Zoogloea ramigera
Reaction
Reaction
Commentary
Organism
2 acetyl-CoA = CoA + acetoacetyl-CoA
bi bi ping pong mechanism
Zoogloea ramigera
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
91
-
-
Zoogloea ramigera
Storage Stability
Storage Stability
Organism
-20°C, 50 mM Tris-HCl, pH 8.0, 0.5 mM dithiothreitol, glycerol
Zoogloea ramigera
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA + acetoacetyl-CoA
-
487668
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487668
Zoogloea ramigera
?
CoA + acetoacetyl-CoA
-
487668
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487668
Zoogloea ramigera
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 44000, SDS-PAGE
Zoogloea ramigera
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
condensation
Zoogloea ramigera
8.5
-
thiolysis
Zoogloea ramigera
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
approx. 80% of maximal activity at pH 6.0, approx. 40% at pH 9.0
Zoogloea ramigera
6.5
9.2
approx. 50% of maximal activity at pH 6.5 and pH 9.2, thiolysis
Zoogloea ramigera
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
5,5'-dithiobis(2-nitrobenzoate)
0.4 mM, 87% inhibition
Zoogloea ramigera
iodoacetamide
0.4 mM, 36% inhibition
Zoogloea ramigera
N-ethylmaleimide
0.4 mM, 53% inhibition
Zoogloea ramigera
NADH
inhibition of thiolysis
Zoogloea ramigera
NADPH
-
Zoogloea ramigera
p-chloromercuribenzoate
0.4 mM, 95% inhibition
Zoogloea ramigera
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0085
-
CoA
-
Zoogloea ramigera
0.01
-
acetoacetyl-CoA
-
Zoogloea ramigera
0.33
-
acetyl-CoA
-
Zoogloea ramigera
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
44000
-
4 * 44000, SDS-PAGE
Zoogloea ramigera
190000
-
gel filtration
Zoogloea ramigera
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA + acetoacetyl-CoA
Zoogloea ramigera
-
acetyl-CoA + acetyl-CoA
-
Zoogloea ramigera
?
Purification (Commentary) (protein specific)
Commentary
Organism
protamine sulfate, DEAE-cellulose, phosphocellulose, Sephadex G-200
Zoogloea ramigera
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
91
-
-
Zoogloea ramigera
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 50 mM Tris-HCl, pH 8.0, 0.5 mM dithiothreitol, glycerol
Zoogloea ramigera
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA + acetoacetyl-CoA
-
487668
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487668
Zoogloea ramigera
?
CoA + acetoacetyl-CoA
-
487668
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487668
Zoogloea ramigera
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 44000, SDS-PAGE
Zoogloea ramigera
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
condensation
Zoogloea ramigera
8.5
-
thiolysis
Zoogloea ramigera
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
approx. 80% of maximal activity at pH 6.0, approx. 40% at pH 9.0
Zoogloea ramigera
6.5
9.2
approx. 50% of maximal activity at pH 6.5 and pH 9.2, thiolysis
Zoogloea ramigera
Other publictions for EC 2.3.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736252
Kim
Crystal structure and biochemi ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Int. J. Biol. Macromol.
82
425-431
2016
-
-
1
1
8
-
-
1
-
-
-
4
-
2
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
8
-
-
-
-
1
-
-
-
4
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
735372
Janardan
Structural characterization of ...
Mycolicibacterium smegmatis
Acta Crystallogr. Sect. D
71
2479-2493
2015
-
-
1
1
-
-
-
-
1
-
-
2
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
735404
Kim
Purification, crystallization ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Acta Crystallogr. Sect. F
71
758-762
2015
-
-
1
1
-
-
-
1
-
1
2
6
-
5
-
-
1
-
-
-
-
-
6
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
1
2
6
-
-
-
1
-
-
-
-
6
1
1
-
-
1
1
-
-
-
-
1
1
-
1
1
735609
Kim
Crystal structure and biochemi ...
Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
Biochem. Biophys. Res. Commun.
459
547-552
2015
-
-
1
1
7
-
-
-
-
-
-
6
-
3
-
-
1
-
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
-
-
-
-
6
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
736168
Zhong
Two different subcellular-loca ...
Magnaporthe oryzae, Magnaporthe oryzae Ku80
Fungal Genet. Biol.
83
58-67
2015
-
-
-
-
-
-
-
-
3
-
-
2
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
736542
Yun
Production of (S)-3-hydroxybut ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae BY4741
J. Biotechnol.
209
23-30
2015
-
-
1
-
-
-
-
-
-
-
-
2
-
59
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736771
Isezaki
Dictyostelium acetoacetyl-CoA ...
Dictyostelium discoideum, Dictyostelium discoideum Ax-3
Microbiology
161
1471-1484
2015
-
-
-
-
-
-
-
-
6
-
1
2
-
5
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736876
Kim
Redox-switch regulatory mechan ...
Clostridium acetobutylicum
Nature Commun.
6
8410
2015
-
1
1
1
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736941
Lin
Clonorchis sinensis acetoacety ...
Clonorchis sinensis
Parasit. Vectors
8
125
2015
-
-
1
-
-
-
-
-
-
-
1
1
-
3
-
-
1
-
-
4
-
-
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
4
-
-
1
1
1
-
-
-
1
-
-
-
1
1
1
1
-
-
726748
Hawkins
Conversion of 4-hydroxybutyrat ...
Metallosphaera sedula, Metallosphaera sedula DSM 5348
Appl. Environ. Microbiol.
80
2536-2545
2014
-
-
1
-
-
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
1
1
-
-
-
-
1
1
-
1
1
726744
Hou
Haloarchaeal-type beta-ketothi ...
Haloferax mediterranei, Haloferax mediterranei DSM 1411
Appl. Environ. Microbiol.
79
5104-5111
2013
-
1
-
-
1
-
-
-
-
-
-
4
-
5
-
-
-
-
-
-
-
-
14
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
14
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
737008
Vishwakarma
-
Molecular cloning, biochemical ...
Bacopa monnieri
Plant Mol. Biol. Rep.
31
547-557
2013
-
-
1
-
-
-
1
2
-
2
2
1
-
1
-
-
1
-
-
6
2
-
2
1
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
2
-
2
2
1
-
-
-
1
-
6
2
-
2
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
720708
Jin
Reverse genetic characterizati ...
Arabidopsis thaliana
Plant J.
70
1015-1032
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
719606
Tanaka
Expression, identification and ...
Dictyostelium discoideum
Int. J. Biol. Sci.
7
9-17
2011
-
-
1
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
1
-
1
1
-
-
2
-
1
1
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
1
-
1
1
-
-
2
-
1
1
-
1
-
-
-
-
-
-
720134
Soto
Acetoacetyl-CoA thiolase regul ...
Medicago sativa
J. Exp. Bot.
62
5699-5711
2011
-
-
1
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
727751
Ramos-Vera
Identification of missing gene ...
Pyrobaculum neutrophilum, Pyrobaculum neutrophilum DSM 2338
J. Bacteriol.
193
1201-1211
2011
-
-
1
-
-
-
-
1
-
-
1
2
-
2
-
-
1
-
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
2
-
-
-
1
-
-
1
-
4
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
706822
Thuemmler
Different clinical presentatio ...
Homo sapiens
Tohoku J. Exp. Med.
220
27-31
2010
-
-
-
-
1
-
-
-
1
-
-
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
704027
Fujii
Molecular and functional chara ...
Ostrinia scapulalis
Insect Biochem. Mol. Biol.
40
74-78
2009
-
1
1
-
-
-
-
-
-
-
3
1
-
5
-
-
-
-
-
7
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
7
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
704063
Dyer
Cloning, expression and purifi ...
Helianthus annuus
Int. J. Biol. Sci.
5
736-744
2009
-
-
1
-
-
-
-
-
1
-
1
-
-
6
-
-
1
-
-
3
2
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
3
2
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
706809
Battilana
The 1-deoxy-D: -xylulose 5-pho ...
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
Theor. Appl. Genet.
118
653-669
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
689028
Fukao
A novel single-base substituti ...
Homo sapiens
Mol. Genet. Metab.
94
417-421
2008
-
-
-
-
1
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
702812
Reddick
The mmgA gene from Bacillus su ...
Bacillus subtilis
Biotechnol. Lett.
30
1045-1050
2008
-
-
1
-
-
-
-
2
-
-
2
-
-
3
-
-
1
-
-
-
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
1
-
-
-
-
1
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
703818
Ahumada
-
Characterisation of the gene f ...
Arabidopsis thaliana
Funct. Plant Biol.
35
1100-1111
2008
-
-
1
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676035
Sakurai
Kinetic and expression analyse ...
Homo sapiens
Mol. Genet. Metab.
90
370-378
2007
-
-
-
-
9
-
1
4
1
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
4
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
685147
Haapalainen
Crystallographic and kinetic s ...
Homo sapiens
Biochemistry
46
4305-4321
2007
-
-
-
1
-
-
-
-
2
2
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
2
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686989
Santhanam
Impairment of mitochondrial ac ...
Homo sapiens
Gut
56
1543-1549
2007
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676336
Treberg
Activation of liver carnitine ...
Squalus acanthias
Physiol. Biochem. Zool.
79
899-908
2006
-
-
-
-
-
-
-
-
2
-
-
1
-
8
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659726
Kursula
High resolution crystal struct ...
Escherichia coli, Homo sapiens, Zoogloea ramigera
J. Mol. Biol.
347
189-201
2005
-
-
1
1
-
-
-
-
1
-
-
3
-
6
-
-
1
3
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748648
Pantazaki
A thermostable beta-ketothiol ...
Thermus thermophilus
Mol. Cell. Biochem.
269
27-36
2005
1
-
-
-
-
-
3
3
-
-
2
1
1
1
-
-
1
-
1
-
1
-
3
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
3
-
-
2
1
1
-
-
1
1
-
1
-
3
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
658880
Fukao
Single base substitutions at t ...
Homo sapiens
Hum. Mutat.
21
587-592
2003
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486430
Oeljeklaus
Glyoxysomal acetoacetyl-CoA th ...
Helianthus annuus
Planta
214
597-607
2002
-
-
-
-
-
1
-
2
2
-
2
1
-
2
-
-
1
-
-
2
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
2
-
2
1
-
-
-
1
-
2
1
-
2
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
487701
Kursula
The catalytic cycle of biosynt ...
Zoogloea ramigera
Biochemistry
41
15543-15556
2002
-
-
1
1
2
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487702
Hedl
Enterococcus faecalis acetoace ...
Enterococcus faecalis
J. Bacteriol.
184
2116-2122
2002
-
-
1
-
-
-
1
3
-
-
2
1
-
6
-
-
1
-
-
-
-
-
2
1
1
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
3
-
-
2
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
487703
Liu
Purification and characterizat ...
Halobacterium sp., Halobacterium sp. ZP-6
Extremophiles
6
97-102
2002
2
-
-
-
-
1
-
-
-
-
3
2
-
5
-
-
1
-
-
-
1
2
4
1
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
3
2
-
-
-
1
-
-
1
2
4
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
486875
Lobo
A Streptomyces collinus thiola ...
Streptomyces collinus
Biochemistry
40
11955-11964
2001
-
-
1
-
1
-
1
3
-
-
2
1
-
1
-
-
1
-
-
-
1
-
2
1
-
-
1
3
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
3
-
-
2
1
-
-
-
1
-
-
1
-
2
1
-
-
1
3
-
-
-
-
-
-
-
-
-
-
487699
Antonenkov
Identification, purification a ...
Rattus norvegicus
Eur. J. Biochem.
267
2981-2990
2000
-
-
-
-
-
-
-
6
2
-
2
1
-
2
-
-
1
-
-
2
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
2
-
2
1
-
-
-
1
-
2
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487698
Modis
A biosynthetic thiolase in com ...
Zoogloea ramigera
Structure
7
1279-1290
1999
-
-
1
1
-
-
-
-
-
-
2
1
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
486436
Kanayama
Genetic evaluation of physiolo ...
Candida tropicalis
J. Bacteriol.
180
690-698
1998
-
-
-
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487697
Honda
Regulation of early cholestero ...
Rattus norvegicus
Hepatology
27
154-159
1998
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487694
Kanayama
Expression of acetoacetyl-CoA ...
Candida tropicalis, Rhizobium sp.
J. Biochem.
122
616-621
1997
-
-
1
-
-
-
-
12
8
-
3
2
-
4
-
-
1
-
-
-
1
-
4
1
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
12
8
-
3
2
-
-
-
1
-
-
1
-
4
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
487695
Kim
Acetyl coenzyme A acetyltransf ...
Rhizobium sp.
Appl. Environ. Microbiol.
63
3432-3437
1997
-
-
-
-
-
-
3
4
-
3
2
1
-
3
-
-
1
-
-
2
1
3
2
1
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
3
2
4
-
3
2
1
-
-
-
1
-
2
1
3
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
487693
Heijden
-
Biosynthesis of 3S-hydroxy-3-m ...
Catharanthus roseus
Plant Physiol. Biochem.
32
807-812
1994
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487683
Palmer
Biosynthetic thiolase from Zoo ...
Zoogloea ramigera
J. Biol. Chem.
266
8369-8375
1991
-
-
-
-
1
-
3
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
3
2
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487691
O'Connell
Purification and characterizat ...
Caulobacter vibrioides
J. Bacteriol.
172
997-1004
1990
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
487692
Anderson
-
The reaction of acetyldithio-C ...
Zoogloea ramigera
J. Biol. Chem.
266
6255-6261
1990
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
288676
Kurihara
Peroxisomal acetoacetyl-CoA th ...
Candida tropicalis
J. Biochem.
106
474-478
1989
-
-
-
-
-
-
-
1
4
-
1
1
-
4
-
-
1
-
-
2
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
4
-
1
1
-
-
-
1
-
2
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487688
Greenspan
The inhibition of cytoplasmic ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
163
548-553
1989
-
-
-
-
-
-
1
-
2
-
-
1
-
3
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
2
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487684
Wiesenborn
Thiolase from Clostridium acet ...
Clostridium acetobutylicum
Appl. Environ. Microbiol.
54
2717-2722
1988
-
-
-
-
-
-
5
3
-
-
1
1
-
1
-
-
1
1
-
-
1
1
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
3
-
-
1
1
-
-
-
1
-
-
1
1
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
487680
Davis
Biosynthetic thiolase from zoo ...
Zoogloea ramigera
J. Biol. Chem.
262
82-89
1987
-
-
-
-
-
1
1
7
-
-
1
1
-
1
-
-
1
-
-
-
2
-
9
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
7
-
-
1
1
-
-
-
1
-
-
2
-
9
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
487681
Davis
Biosynthetic thiolase from Zoo ...
Zoogloea ramigera
J. Biol. Chem.
262
90-96
1987
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487682
Peoples
Biosynthetic thiolase from Zoo ...
Zoogloea ramigera
J. Biol. Chem.
262
97-102
1987
-
-
1
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
4
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487687
Suzuki
Acetoacetyl-CoA thiolase of Br ...
Bradyrhizobium japonicum
Arch. Biochem. Biophys.
254
272-281
1987
-
-
-
-
-
3
4
3
-
-
3
1
-
2
-
-
1
1
-
1
1
1
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
3
-
4
-
3
-
-
3
1
-
-
-
1
-
1
1
1
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
286047
Sliwkowski
Simultaneous single-step purif ...
Clostridium kluyveri
Anal. Biochem.
141
344-347
1984
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
487672
Hartmanis
Isolation of a selenium-contai ...
Clostridium kluyveri
Proc. Natl. Acad. Sci. USA
79
4912-4916
1982
-
-
-
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486408
Olowe
Regulation of thiolases from p ...
Sus scrofa
Eur. J. Biochem.
109
425-429
1980
-
-
-
-
-
-
2
-
1
-
-
1
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487676
Schwabe
Immunochemical aspects, molecu ...
Rattus norvegicus
Biochim. Biophys. Acta
575
112-120
1979
-
-
-
-
-
-
-
1
1
-
2
1
-
2
-
-
1
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
2
1
-
-
-
1
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487668
Nishimura
Purification and properties of ...
Zoogloea ramigera
Arch. Microbiol.
116
21-27
1978
-
-
-
-
-
-
6
3
-
-
2
1
-
1
-
-
1
1
-
-
1
1
2
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
3
-
-
2
1
-
-
-
1
-
-
1
1
2
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
487674
Bloxham
Synthesis of chloromethyl keto ...
Sus scrofa
Biochem. J.
175
999-1011
1978
-
-
-
-
-
-
9
-
-
-
-
1
-
3
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
9
8
-
-
-
-
1
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487677
Jonas
Acetyl-CoA acetyltransferase f ...
Bos taurus
Biochim. Biophys. Acta
527
379-390
1978
-
-
-
-
-
-
-
-
1
-
6
1
-
2
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
1
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487690
Duncombe
Molecular and catalytic proper ...
Escherichia coli
Arch. Biochem. Biophys.
176
159-170
1976
-
-
-
-
-
-
6
1
-
-
3
1
-
1
1
-
-
-
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
1
-
-
3
1
-
1
-
-
-
-
1
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486410
Feigenbaum
Thiolases of Escherichia coli: ...
Escherichia coli
J. Bacteriol.
122
407-411
1975
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487669
Clinkenbeard
-
Cytosolic acetoacetyl-CoA thio ...
Gallus gallus
Methods Enzymol.
35 B
167-173
1975
-
-
-
-
-
-
1
3
1
-
3
1
-
1
-
-
1
-
-
1
2
1
2
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
3
1
-
3
1
-
-
-
1
-
1
2
1
2
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
487675
Huth
On the mechanism of ketogenesi ...
Bos taurus
Eur. J. Biochem.
59
475-489
1975
-
-
-
1
-
-
2
6
-
-
-
1
-
1
-
-
1
1
-
1
-
1
2
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
1
-
-
-
2
2
6
-
-
-
1
-
-
-
1
-
1
-
1
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
487673
Middleton
The kinetic mechanism and prop ...
Rattus norvegicus
Biochem. J.
139
109-121
1974
-
-
-
-
-
-
3
-
1
-
2
1
-
1
-
-
1
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
1
-
1
-
2
1
-
-
-
1
-
1
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487678
Huth
Multiple mitochondrial forms o ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
56
1069-1077
1974
-
-
-
-
-
-
1
4
1
-
-
1
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
4
1
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487670
Holland
Inactivation of pig heart thio ...
Sus scrofa
Biochemistry
12
3309-3315
1973
-
-
-
-
-
-
7
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
7
2
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487671
Clinkenbeard
Molecular and catalytic proper ...
Gallus gallus
J. Biol. Chem.
248
2275-2284
1973
-
-
-
-
-
-
-
-
1
-
2
1
-
1
-
-
1
-
-
1
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
1
1
1
2
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
487666
Stern
Enzymes of ketone body metabol ...
Bos taurus
J. Biol. Chem.
235
313-317
1960
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
487667
Lynen
Enzymes of fatty acid metaboli ...
Clostridium kluyveri, Sus scrofa
Biochim. Biophys. Acta
12
299-314
1953
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-