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Literature summary for 2.3.1.9 extracted from
- Simultaneous single-step purification of thiolase and NADP-dependent 3-hydroxybutyryl-CoA dehydrogenase from Clostridium kluyveri (1984), Anal. Biochem., 141, 344-347.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
4 * 41000, SDS-PAGE | Clostridium kluyveri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | Clostridium kluyveri | - |
acetyl-CoA + acetyl-CoA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium kluyveri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CM-Sephadex | Clostridium kluyveri |
| single-step purification | Clostridium kluyveri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 115 | - |
- |
Clostridium kluyveri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA + acetoacetyl-CoA | - |
Clostridium kluyveri | acetyl-CoA + acetyl-CoA | - |
? | |
| CoA + acetoacetyl-CoA | - |
Clostridium kluyveri | acetyl-CoA + acetyl-CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 41000, SDS-PAGE | Clostridium kluyveri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Clostridium kluyveri |
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