Literature summary for 2.3.1.87 extracted from

Szewczuk, L.M.; Tarrant, M.K.; Sample, V.; Drury, W.J.; Zhang, J.; Cole, P.A.
Analysis of serotonin N-acetyltransferase regulation in vitro and in live cells using protein semisynthesis (2008), Biochemistry, 47, 10407-10419.

Search for more literature for 2.3.1.87

Activating Compound

Activating Compound	Comment	Organism	Structure
14-3-3 protein	14-3-3zeta, interaction with the phosphorylated enzyme activates activity, binding analysis, overview	Homo sapiens
additional information	the enzyme is activated by Ser and Thr phosphorylations, e.g. AANAT Thr31 phosphorylation on its own can enhance catalytic efficiency up to 7fold	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of truncation mutants in Escherichia coli strain BL21(DE3)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of truncation mutants AANAT 1-197(DELTAC8) and GST-AANAT 34-197 (DELTAN33, DELTAC8). Production of partially and differentially phosphorylated enzymes by semiprotein synthesis, overview	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetics of recombinant partially phosphorylated wild-type and mutant enzymes, overview	Homo sapiens
0.06	-	serotonin	wild-type enzyme	Homo sapiens
0.096	-	serotonin	wild-type enzyme with 14-3-3 protein	Homo sapiens
0.106	-	serotonin	wild-type enzyme with protein kinase A	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	enzyme light and diurnal regulation involves phosphorylation on key AANAT Ser and Thr residues, which results in 14-3-3 protein recruitment and changes in catalytic activity and protein stability	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation on key AANAT Ser and Thr residues by protein kinase A results in 14-3-3 protein recruitment and changes in catalytic activity and protein stability. AANAT Thr31 phosphorylation on its own can enhance catalytic efficiency up to 7fold, but the catalytic profile is largely abolished by double phosphorylation at Thr31 and Ser205. Influence of phosphorylations on enzyme activity, overview	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
pineal gland	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + serotonin	-	Homo sapiens	CoA + N-acetylserotonin	-	?
additional information	enzyme light and diurnal regulation involves phosphorylation on key AANAT Ser and Thr residues, which results in 14-3-3 protein recruitment and changes in catalytic activity and protein stability	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
AANAT	-	Homo sapiens
arylalkylamine N-acetyltransferase	-	Homo sapiens
serotonin N-acetyltransferase	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
17.3	-	serotonin	wild-type enzyme	Homo sapiens
20.3	-	serotonin	wild-type enzyme with 14-3-3 protein	Homo sapiens
25.9	-	serotonin	wild-type enzyme with protein kinase A	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	AANAT is a key circadian rhythm enzyme that drives the nocturnal production of melatonin in the pineal	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
210	-	serotonin	wild-type enzyme with 14-3-3 protein	Homo sapiens
240	-	serotonin	wild-type enzyme with protein kinase A	Homo sapiens
290	-	serotonin	wild-type enzyme	Homo sapiens

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Release 2023.1 (January 2023)