Literature summary for 2.3.1.87 extracted from

Choi, B.H.; Chae, H.D.; Park, T.J.; Oh, J.; Lim, J.; Kang, S.S.; Ha, H.; Kim, K.T.
Protein kinase C regulates the activity and stability of serotonin N-acetyltransferase (2004), J. Neurochem., 90, 442-454.

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Activating Compound

Activating Compound	Comment	Organism	Structure
forskolin	decreases KM-value for tryptamine by 32%	Rattus norvegicus
phorbol 12-myristate 13-acetate	decreases KM-value for tryptamine by 44%	Rattus norvegicus

Protein Variants

Protein Variants	Comment	Organism
S192V	decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells	Rattus norvegicus
T127V	decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells	Rattus norvegicus
T29V	mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells	Rattus norvegicus
T29V/S203G	mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.291	-	tryptamine	pH 6.8, 37°C, mutant enzyme S192V	Rattus norvegicus
0.317	-	tryptamine	pH 6.8, 37°C, mutant enzyme T29V/S203G	Rattus norvegicus
0.344	-	tryptamine	pH 6.8, 37°C, mutant enzyme T127V	Rattus norvegicus
0.361	-	tryptamine	pH 6.8, 37°C, wild-type enzyme	Rattus norvegicus
0.413	-	tryptamine	pH 6.8, 37°C, mutant enzyme T29V	Rattus norvegicus
0.417	-	tryptamine	pH 6.8, 37°C, mutant enzyme S203G	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylated at Thr29 by protein kinase C activation through the alpha1-adrenergic receptor in rat pineal glands. Phosphorylation may contribute to the stability and activity of the enzyme	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
pineal gland	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetyl-CoA + tryptamine	-	Rattus norvegicus	CoA + N-acetyltryptamine	-	?

Synonyms

Synonyms	Comment	Organism
AANAT	-	Rattus norvegicus
serotonin N-acetyltransferase	-	Rattus norvegicus

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Release 2023.1 (January 2023)