Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.3.1.87 extracted from

  • Zilberman-Peled, B.; Benhar, I.; Coon, S.L.; Ron, B.; Gothilf, Y.
    Duality of serotonin-N-acetyltransferase in the gilthead seabream (Sparus aurata): molecular cloning and characterization of recombinant enzymes (2004), Gen. Comp. Endocrinol., 138, 139-147.
    View publication on PubMed
Search for more literature for 2.3.1.87

Activating Compound

Activating Compound Comment Organism Structure
phosphate optimal activity at 0.1 M Sparus aurata
phosphate optimal activity at 0.3 M Sparus aurata

Cloned(Commentary)

Cloned (Comment) Organism
-
 Sparus aurata

Inhibitors

Inhibitors Comment Organism Structure
Melatonin IC50: 0.26 mM Sparus aurata
additional information no inhibition by serotonin or tryptamin above 1 mM. No inhibition by melatonin Sparus aurata
N-acetylserotonin IC50: 0.68 mM; very slightly Sparus aurata
serotonin above 1 mM Sparus aurata
tryptamine above 1 mM Sparus aurata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 serotonin
-
 Sparus aurata
0.1
-
 tryptamine
-
 Sparus aurata
0.34
-
 Phenylethylamine
-
 Sparus aurata
0.62
-
 acetyl-CoA
-
 Sparus aurata
1.11
-
 acetyl-CoA pH 6.8, 27°C Sparus aurata
1.25
-
 tyramine
-
 Sparus aurata
1.4
-
 tryptamine pH 6.8, 27°C Sparus aurata
2.05
-
 serotonin pH 6.8, 27°C Sparus aurata

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl optimal activity at 0.5 M Sparus aurata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sparus aurata AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin ?
-
 ?
additional information Sparus aurata AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sparus aurata Q68SL6
-
-
Sparus aurata Q68SL7
-
-

Source Tissue

Source Tissue Comment Organism Textmining
photoreceptor
-
 Sparus aurata
-
pineal gland
-
 Sparus aurata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + phenylethylamine
-
 Sparus aurata CoA + N-acetyl-phenylethylamine
-
 ?
acetyl-CoA + serotonin
-
 Sparus aurata CoA + N-acetylserotonin
-
 ?
acetyl-CoA + serotonin AANAT2 acetylates serotonin about 10times faster than AANAT1 Sparus aurata CoA + N-acetylserotonin
-
 ?
acetyl-CoA + tryptamine
-
 Sparus aurata CoA + N-acetyltryptamine
-
 ?
acetyl-CoA + tyramine
-
 Sparus aurata CoA + N-acetyltyramine
-
 ?
additional information AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin Sparus aurata ?
-
 ?
additional information AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role Sparus aurata ?
-
 ?
additional information insignificant activity in presence of phenylethylamine or tyramine Sparus aurata ?
-
 ?

Synonyms

Synonyms Comment Organism
AANAT1
-
 Sparus aurata
AANAT2
-
 Sparus aurata
serotonin-N-acetyltransferase
-
 Sparus aurata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
-
 Sparus aurata
36
-
-
 Sparus aurata

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 40 0°C: about 55% of maximal activity, 47°C: about 50% of maximal activity Sparus aurata
20 47 20°C: about 40% of maximal activity, 47°C: about 35% of maximal activity Sparus aurata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Sparus aurata

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.26
-
 IC50: 0.26 mM Sparus aurata Melatonin
0.68
-
 IC50: 0.68 mM Sparus aurata N-acetylserotonin