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Literature summary for 2.3.1.86 extracted from

  • Henritzi, S.; Fischer, M.; Grininger, M.; Oreb, M.; Boles, E.
    An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol in Saccharomyces cerevisiae (2018), Biotechnol. Biofuels, 11, 150.
    View publication on PubMed
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Application

Application Comment Organism
synthesis short-chain acyl-CoA producing yeast Fas1 mutant R1834K/Fas2 fatty acid synthase variant is expressed together with carboxylic acid reductase from Mycobacterium marinum and phosphopantetheinyl transferase Sfp from Bacillus subtilis in a Saccharomyces cerevisiae DELTAfas1 DELTAfas2 DELTAfaa2 mutant strain. The synthesized octanoyl-CoA is endogenously converted to 1-octanol up to a titer of 26.0 mg/l in a 72-h fermentation. When octanoic acid is supplied externally to the yeast cells, it can be efficiently converted to 1-octanol. Additional overexpression of aldehyde reductase Ahr from Escherichia coli nearly completely prevents accumulation of octanoic acid and increases 1-octanol titers up to 49.5 mg/l. 1-octanol acts inhibitive before secretion Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
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 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
R1834K mutation in Fas1 gene. Mutant produces short-chain acyl-CoAs Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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