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Literature summary for 2.3.1.86 extracted from

  • Vance, D.E.; Mitsuhashi, O.; Bloch, K.
    Purification and properties of the fatty acid synthetase from Mycobacterium phlei (1973), J. Biol. Chem., 248, 2303-2309.
    View publication on PubMed
Search for more literature for 2.3.1.86

Activating Compound

Activating Compound Comment Organism Structure
3-O-methylmannose 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA Mycolicibacterium phlei
6-O-methylglucose 0.1 mM, lowers Km 4fold, activation by relieving product inhibition by binding long-chain acyl-CoA Mycolicibacterium phlei

General Stability

General Stability Organism
low ionic strength, 0.005 M, leads to dissociation into subunits, partially reactivated by increasing the ionic strength to 0.5 M Mycolicibacterium phlei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
 malonyl-CoA in presence of polysaccharide Mycolicibacterium phlei
0.04
-
 malonyl-CoA
-
 Mycolicibacterium phlei
0.09
-
 acetyl-CoA in presence of polysaccharide Mycolicibacterium phlei
0.8
-
 acetyl-CoA
-
 Mycolicibacterium phlei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1390000
-
 sedimentation velocity data Mycolicibacterium phlei

Organism

Organism UniProt Comment Textmining
Mycolicibacterium phlei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Mycolicibacterium phlei

Renatured (Commentary)

Renatured (Comment) Organism
low concentration of phosphate buffer causes dissociation into inactive species, reaggregation and reactivation can be partially achieved by dialysis against 0.5 M phosphate buffer Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.375
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ primers instead of acetyl-CoA: propionyl-CoA, butyryl-CoA or hexanoyl-CoA Mycolicibacterium phlei palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Mycolicibacterium phlei
NADPH requirement Mycolicibacterium phlei