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Literature summary for 2.3.1.86 extracted from

  • Wakil, S.J.; Stoops, J.K.; Joshi, V.C.
    Fatty acid synthesis and its regulation (1983), Annu. Rev. Biochem., 52, 537-579.
    View publication on PubMed
Search for more literature for 2.3.1.86

Cloned(Commentary)

Cloned (Comment) Organism
transformation technique, plasmid YEpFAS2 transformed to and expressed in Escherichia coli maxi-cells Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
1,3-Dibromo-2-propanone
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Mycolicibacterium smegmatis
additional information
-
 alpha6beta6-complex of multifunctional subunits Saccharomyces cerevisiae
additional information
-
 amino acid sequence of acyl-carrier-protein Saccharomyces cerevisiae
180000
-
 alpha6,beta6, 6 * 185000 + 6 * 180000, SDS-PAGE Saccharomyces cerevisiae
185000
-
 alpha6,beta6, 6 * 185000 + 6 * 180000, SDS-PAGE Saccharomyces cerevisiae
203000
-
 alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
213000
-
 alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
2400000
-
 sedimentation velocity Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH Mycolicibacterium smegmatis multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isolation of alpha- and beta-subunits Saccharomyces cerevisiae
isolation of alpha- and beta-subunits by citraconic and dimethylmaleic anhydride modification Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H+ = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+ structure Saccharomyces cerevisiae
a
acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H+ = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+ reaction mechanism Saccharomyces cerevisiae
a
acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H+ = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+ reaction mechanism Mycolicibacterium smegmatis
a
acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H+ = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+ structure and regulation Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Mycolicibacterium smegmatis palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ stable multifunctional enzyme complex: carries acetyl-CoA and malonyl-CoA transacylase, beta-ketoacyl reductase, beta-hydroxyacyl dehydrase activities on beta-subunits and condensing enzyme, i.e. beta-ketoacyl synthetase, enoylacyl reductase activities and acyl-carrier-protein components on alpha-subunits Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Mycolicibacterium smegmatis ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer alpha6,beta6, 6 * 185000 + 6 * 180000, SDS-PAGE Saccharomyces cerevisiae
dodecamer alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
additional information
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
4'-phosphopantetheine 4.0-5.0 mol per mol enzyme or about 1 mol per 2 subunits Saccharomyces cerevisiae
4'-phosphopantetheine 4.0-5.0 mol per mol enzyme Saccharomyces cerevisiae
4'-phosphopantetheine alpha-subunit bears prosthetic group Saccharomyces cerevisiae
FMN requirement, enoyl reductase activity, associated with beta-subunit, 6 mol per mol synthetase Saccharomyces cerevisiae
NADPH requirement Saccharomyces cerevisiae
NADPH requirement Mycolicibacterium smegmatis