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Literature summary for 2.3.1.86 extracted from
- Studies on the yeast fatty acid synthetase. Subunit composition and structural organization of a large multifunctional enzyme complex (1978), J. Biol. Chem., 253, 4464-4475.
General Stability
| General Stability | Organism |
|---|---|
| PMSF ensures isolation of native enzyme | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methylamine tungstad | inactivation within 24 h | Saccharomyces cerevisiae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
alpha6beta6-complex of multifunctional subunits | Saccharomyces cerevisiae |
| additional information | - |
amino acid composition | Saccharomyces cerevisiae |
| 203000 | - |
alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE | Saccharomyces cerevisiae |
| 213000 | - |
alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE | Saccharomyces cerevisiae |
| 2370000 | - |
sedimentation equilibrium method | Saccharomyces cerevisiae |
| 2400000 | - |
sedimentation velocity | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-CoA + NADH + NADPH | Saccharomyces cerevisiae | multifunctional enzyme involved in yeast fat metabolism | ? | - |
? | |
| acetyl-CoA + malonyl-CoA + NADH + NADPH | Saccharomyces cerevisiae Fleishmann | multifunctional enzyme involved in yeast fat metabolism | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
wild type and protease-negative pep4-mutant | - |
| Saccharomyces cerevisiae Fleishmann | - |
wild type and protease-negative pep4-mutant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isolation of peptides after proteolysis | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| acetyl-CoA + n malonyl-CoA + 2n NADPH + 4n H+ = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+ | structure | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | 4 | - |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 month | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + malonyl-CoA + NADH + NADPH | multifunctional enzyme involved in yeast fat metabolism | Saccharomyces cerevisiae | ? | - |
? | |
| acetyl-CoA + malonyl-CoA + NADH + NADPH | multifunctional enzyme involved in yeast fat metabolism | Saccharomyces cerevisiae Fleishmann | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dodecamer | alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE | Saccharomyces cerevisiae |
| dodecamer | active enzyme centrifugation | Saccharomyces cerevisiae |
| additional information | - |
Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 4'-phosphopantetheine | 4.0-5.0 mol per mol enzyme or about 1 mol per 2 subunits | Saccharomyces cerevisiae | |
| NADPH | requirement | Saccharomyces cerevisiae | |
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