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Literature summary for 2.3.1.86 extracted from

  • Yein, F.; Brown, O.R.
    Comparative inactivation of yeast fatty acid synthetase component enzymes by 100 atmospheres of oxygen (1977), Biochim. Biophys. Acta, 486, 421-428.
    View publication on PubMed
Search for more literature for 2.3.1.86

Activating Compound

Activating Compound Comment Organism Structure
cysteine not Saccharomyces cerevisiae
additional information no activation by DTT Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Oxidation Stability

Oxidation Stability Organism
under 100 atm nitrogen, at 4°C 1-20 h stable, 40% loss of activity after 40 h Saccharomyces cerevisiae
under 100 atm oxygen, at 4°C 1-2 h stable, 48% and 90% loss of activity after 20 h and 40 h, respectively, DTT does not restore activity Saccharomyces cerevisiae

Purification (Commentary)

Purification (Comment) Organism
900fold Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activities of components Saccharomyces cerevisiae
0.062
-
 substrates: acetoacetyl-cysteamine, malonyl-CoA and NADPH Saccharomyces cerevisiae
0.8 0.83 malonyl-CoA Saccharomyces cerevisiae
1.6
-
 substrates: acetoacetyl-CoA, malonyl-CoA and NADPH H Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH requirement Saccharomyces cerevisiae