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Literature summary for 2.3.1.86 extracted from

  • Stoops, J.K.; Wakil, S.J.
    The isolation of the two subunits of yeast fatty acid synthetase (1978), Biochem. Biophys. Res. Commun., 84, 225-231.
    View publication on PubMed
Search for more literature for 2.3.1.86

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 alpha6beta6-complex of multifunctional subunits Saccharomyces cerevisiae
203000
-
 alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
213000
-
 alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
2400000
-
 sedimentation velocity Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isolation of alpha- and beta-subunits Saccharomyces cerevisiae
isolation of alpha- and beta-subunits by citraconic and dimethylmaleic anhydride modification or as 3,4,5,6-tetrahydrophthalate derivatives Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 comparison of partial activities of alpha- and beta-subunits with that of the native complex Saccharomyces cerevisiae
3
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
dodecamer alpha6,beta6, 6 * 213000 + 6 * 203000, Tris-glycine-SDS-PAGE Saccharomyces cerevisiae
additional information
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH requirement Saccharomyces cerevisiae