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Literature summary for 2.3.1.86 extracted from

  • Wieland, F.; Renner, L.; Verfurth, C.; Lynen, F.
    Studies on the multi-enzyme complex of yeast fatty-acid synthetase. Reversible dissociation and isolation of two polypeptide chains (1979), Eur. J. Biochem., 94, 189-197.
    View publication on PubMed
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General Stability

General Stability Organism
acylation of free protein amino groups leads to reversible dissociation into subunits Saccharomyces cerevisiae
low ionic strength leads to inactivation Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Saccharomyces cerevisiae
2300000
-
 analytical ultracentrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 brewer's yeast
-

Purification (Commentary)

Purification (Comment) Organism
isolation of alpha- and beta-subunits by acylation Saccharomyces cerevisiae

Renatured (Commentary)

Renatured (Comment) Organism
hydrolysis under mild acidic condition leads to unmodified subunits, which can be reconstituted to form a complex displaying about 60% of the original activity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.25 1.75 substrate: C2-unit Saccharomyces cerevisiae
2.5 3.5 NADPH Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-15°C, stable in 90% ammonium sulfate solution containing 0.1 M potassium phosphate, pH 6.5, and 1 mM DTT Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
additional information
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADPH requirement Saccharomyces cerevisiae