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Literature summary for 2.3.1.86 extracted from

  • Schweizer, E.; Kniep, B.; Castorph, H.; Holzner, U.
    Pantetheine-free mutants of the yeast fatty-acid-synthetase complex (1973), Eur. J. Biochem., 39, 353-362.
    View publication on PubMed
Search for more literature for 2.3.1.86

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 MW of components Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae X-2180-A multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 wild-type haploid strain X2180-1A and 52 fas-mutant strains
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae X-2180-A
-
-
-

Purification (Commentary)

Purification (Comment) Organism
mutants Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 data of partial reactions of fatty acid synthetase, different fas-mutants of Saccharomyces cerevisiae Saccharomyces cerevisiae
0.53
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+
-
 Saccharomyces cerevisiae X-2180-A palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?
acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae X-2180-A ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 beta-ketoacyl synthetase activity, assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
4'-phosphopantetheine requirement, pantetheinate-free mutants have no beta-ketoacyl synthetase activity Saccharomyces cerevisiae
NADPH requirement Saccharomyces cerevisiae