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Literature summary for 2.3.1.85 extracted from

  • Witkowski, A.; Joshi, A.K.; Smith, S.
    Characterization of the beta-carbon processing reactions of the mammalian cytosolic fatty acid synthase: role of the central core (2004), Biochemistry, 43, 10458-10466.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
G1886F beta-ketoreductase activity, 130fold increase in Km-value Rattus norvegicus
G1888A beta-ketoreductase activity, 25fold increase in Km-value Rattus norvegicus
R1508A similar activity as wild-type in overall reaction, analysis of partial reactions Rattus norvegicus
R1508D 14% of wild-type activity in overall reaction, analysis of partial reactions Rattus norvegicus
R1508K 58% of wild-type activity in overall reaction, analysis of partial reactions Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
NADPH wild-type, enoylreductase activity Rattus norvegicus
0.02
-
NADPH wild-type, beta-ketoreductase activity Rattus norvegicus
0.121
-
NADH wild-type, enoylreductase activity Rattus norvegicus
0.5
-
NADPH wild-type, beta-ketoreductase activity Rattus norvegicus
0.9
-
NADPH mutant G1888A, beta-ketoreductase activity Rattus norvegicus
2.7
-
NADPH mutant G1886F, beta-ketoreductase activity Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+
-
Rattus norvegicus palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O
-
?
acetyl-CoA + malonyl-CoA + NADH
-
Rattus norvegicus palmitate + CoA + CO2 + NAD+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
NADH wild-type, enoylreductase activity Rattus norvegicus
1.1
-
NADPH mutant G1886F, beta-ketoreductase activity Rattus norvegicus
1.1
-
NADH wild-type, beta-ketoreductase activity Rattus norvegicus
7.3
-
NADPH mutant G1888A, beta-ketoreductase activity Rattus norvegicus
9.8
-
NADPH wild-type, enoylreductase activity Rattus norvegicus
73
-
NADPH wild-type, beta-ketoreductase activity Rattus norvegicus