Protein Variants | Comment | Organism |
---|---|---|
G1886F | beta-ketoreductase activity, 130fold increase in Km-value | Rattus norvegicus |
G1888A | beta-ketoreductase activity, 25fold increase in Km-value | Rattus norvegicus |
R1508A | similar activity as wild-type in overall reaction, analysis of partial reactions | Rattus norvegicus |
R1508D | 14% of wild-type activity in overall reaction, analysis of partial reactions | Rattus norvegicus |
R1508K | 58% of wild-type activity in overall reaction, analysis of partial reactions | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
NADPH | wild-type, enoylreductase activity | Rattus norvegicus | |
0.02 | - |
NADPH | wild-type, beta-ketoreductase activity | Rattus norvegicus | |
0.121 | - |
NADH | wild-type, enoylreductase activity | Rattus norvegicus | |
0.5 | - |
NADPH | wild-type, beta-ketoreductase activity | Rattus norvegicus | |
0.9 | - |
NADPH | mutant G1888A, beta-ketoreductase activity | Rattus norvegicus | |
2.7 | - |
NADPH | mutant G1886F, beta-ketoreductase activity | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ | - |
Rattus norvegicus | palmitate + 8 CoA + 7 CO2 + 14 NADP+ + 6 H2O | - |
? | |
acetyl-CoA + malonyl-CoA + NADH | - |
Rattus norvegicus | palmitate + CoA + CO2 + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
NADH | wild-type, enoylreductase activity | Rattus norvegicus | |
1.1 | - |
NADPH | mutant G1886F, beta-ketoreductase activity | Rattus norvegicus | |
1.1 | - |
NADH | wild-type, beta-ketoreductase activity | Rattus norvegicus | |
7.3 | - |
NADPH | mutant G1888A, beta-ketoreductase activity | Rattus norvegicus | |
9.8 | - |
NADPH | wild-type, enoylreductase activity | Rattus norvegicus | |
73 | - |
NADPH | wild-type, beta-ketoreductase activity | Rattus norvegicus |