Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D195N | mutant cannot be recombinantly expressed | Saccharomyces cerevisiae |
H191A | about 50% decrease in acyl-CoA hydrolase activity | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
gel filtration, protein-detergent complex comprising an Atf1 monomer of 65 kDa associated with the Thesit micelle | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + butanol | - |
Saccharomyces cerevisiae | CoA + butyl acetate | - |
? | |
acetyl-CoA + ethanol | - |
Saccharomyces cerevisiae | CoA + ethyl acetate | - |
? | |
acetyl-CoA + heptanol | - |
Saccharomyces cerevisiae | CoA + heptyl acetate | - |
? | |
acetyl-CoA + hexanol | - |
Saccharomyces cerevisiae | CoA + hexyl acetate | - |
? | |
acetyl-CoA + isoamyl alcohol | - |
Saccharomyces cerevisiae | CoA + isoamyl acetate | - |
? | |
acetyl-CoA + isobutanol | - |
Saccharomyces cerevisiae | CoA + isobutyl acetate | - |
? | |
acetyl-CoA + octanol | - |
Saccharomyces cerevisiae | CoA + octyl acetate | - |
? | |
additional information | Atf1 uses acetyl-CoA to synthesize acetate esters from all of the alcohols tested. Atf1 is specific for acetyl-CoA. Additionally, Atf1 can hydrolyse all of the acyl-CoA substrates tested, with a clear preference for C8 chain lengths | Saccharomyces cerevisiae | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
ATF1 | - |
Saccharomyces cerevisiae |