Protein Variants | Comment | Organism |
---|---|---|
E509A/E512A/E513A | mutation in isoform Atf1, mutated protein shows 84% localization to lipid droplets | Saccharomyces cerevisiae |
L511A/L514A/Y518A/L512A | mutation in isoform Atf1, loss of membrane association | Saccharomyces cerevisiae |
additional information | truncation of amphipathic helices within the N- and C-terminal domains of isoform Atf1 (residues 24-41 and 508-525) impair endoplasmic reticulum localization and membrane association. Mutations of the basic or hydrophobic residues in the N-terminal helix and hydrophobic residues in the C-terminal helix of Atf1 disrupt ER association and subsequent sorting from the ER to lipid droplets. Similar amphipathic helices are found at both ends of isoform Atf2, enabling ER and lipid droplet association. Mutations to the N- and C-terminal helices of Atf2 prevent membrane association | Saccharomyces cerevisiae |
R22A/R23A/R36A | mutation in isoform Atf2, protein is localized in the cytoplasm | Saccharomyces cerevisiae |
W518A/F521/I529A/F532A R36A | mutation in isoform Atf2, protein is localized in the cytoplasm | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | both isoforms Atf1 and Atf2 | Saccharomyces cerevisiae | 5783 | - |
lipid droplet | isoform Atf1 | Saccharomyces cerevisiae | 5811 | - |
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4742 | - |
- |
- |