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Literature summary for 2.3.1.84 extracted from

  • Yoshioka, K.; Hashimoto, N.
    Ester formation by alcohol acetyltransferase from brewer's yeast (1981), Agric. Biol. Chem., 45, 2183-2190.
No PubMed abstract available
Search for more literature for 2.3.1.84

Inhibitors

Inhibitors Comment Organism Structure
2,4,6-Trinitrobenzenesulfonic acid
-
 Saccharomyces uvarum
5,5'-dithiobis(2-nitrobenzoate) 99% inhibition Saccharomyces uvarum
Ag+ 1 mM, 92% inhibition Saccharomyces uvarum
Cd2+ 1 mM, complete inhibition Saccharomyces uvarum
Co2+ 1 mM, 83% inhibition Saccharomyces uvarum
Cu2+ 1 mM, 99% inhibition Saccharomyces uvarum
ergosterol 2 mM, 73% inhibition Saccharomyces uvarum
Hg2+ 1 mM, 98% inhibition Saccharomyces uvarum
iodoacetate
-
 Saccharomyces uvarum
linoleic acid 2 mM, 97% inhibition Saccharomyces uvarum
linolenic acid 2 mM, 97% inhibition Saccharomyces uvarum
monoolein 2 mM, 76% inhibition Saccharomyces uvarum
monostearin 2 mM, 58% inhibition Saccharomyces uvarum
Ni2+ 1 mM, 68% inhibition Saccharomyces uvarum
oleoyl-CoA 2 mM, 87% inhibition Saccharomyces uvarum
p-chloromercuribenzoate 98% inhibition Saccharomyces uvarum
Pb2+ 1 mM, complete inhibition Saccharomyces uvarum
phosphatidylinositol 2 mM, 97% inhibition Saccharomyces uvarum
phosphatidylserine 2 mM, 88% inhibition Saccharomyces uvarum
Tween 60 2 mM, 77% inhibition Saccharomyces uvarum
Tween 80 2 mM, 89% inhibition Saccharomyces uvarum
Zn2+ 1 mM, 97% inhibition Saccharomyces uvarum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane cell membrane bound Saccharomyces uvarum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000
-
 gel filtration Saccharomyces uvarum

Organism

Organism UniProt Comment Textmining
Saccharomyces uvarum
-
 brewery lager yeast
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose 6B, DEAE-Sephadex A50, partially purified Saccharomyces uvarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00729
-
-
 Saccharomyces uvarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanol + acetyl-CoA
-
 Saccharomyces uvarum butyl acetate + CoA
-
 ?
ethanol + acetyl-CoA
-
 Saccharomyces uvarum ethyl acetate + CoA
-
 ?
ethanol + butyryl-CoA
-
 Saccharomyces uvarum ethyl butyrate + CoA
-
 ?
hexanol + acetyl-CoA
-
 Saccharomyces uvarum hexyl acetate + CoA
-
 ?
isoamylalcohol + acetyl-CoA
-
 Saccharomyces uvarum isoamyl acetate + CoA
-
 ?
methanol + acetyl-CoA
-
 Saccharomyces uvarum methyl acetate + CoA
-
 ?
pentanol + acetyl-CoA
-
 Saccharomyces uvarum pentyl acetate + CoA
-
 ?
propanol + acetyl-CoA low activity Saccharomyces uvarum propyl acetate + CoA
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Saccharomyces uvarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 40 25% of maximal activity at 10°C, 50% of maximal activity at 40°C Saccharomyces uvarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 8
-
 Saccharomyces uvarum

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 approx. 40% of maximal activity at pH 5.0, 80% of maximal activity at pH 8.5 Saccharomyces uvarum