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Literature summary for 2.3.1.82 extracted from
- A novel 6'-N-aminoglycoside acetyltransferase, AAC(6)-Ial, from a clinical isolate of Serratia marcescens (2016), Microb. Drug Resist., 22, 103-108 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Serratia marcescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
sisomicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens |  |
| 0.023 | - |
netilmicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.028 | - |
tobramycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.086 | - |
amikacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.322 | - |
kanamycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.399 | - |
arbekacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.404 | - |
isepamicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.47 | - |
neomycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.582 | - |
dibekacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Serratia marcescens | V5YUM6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + amikacin | - |
Serratia marcescens | CoA + N6'-acetylamikacin | - |
? | |
| acetyl-CoA + arbekacin | - |
Serratia marcescens | CoA + 6'-N-acetylarbekacin | - |
? | |
| acetyl-CoA + dibekacin | - |
Serratia marcescens | CoA + N6'-acetyldibekacin | - |
? | |
| acetyl-CoA + isepamicin | - |
Serratia marcescens | CoA + 6'-N-acetylisepamicin | - |
? | |
| acetyl-CoA + kanamycin | - |
Serratia marcescens | CoA + 6'-N-acetylkanamycin | - |
? | |
| acetyl-CoA + neomycin | - |
Serratia marcescens | CoA + 6'-N-acetylneomycin | - |
? | |
| acetyl-CoA + netilmicin | - |
Serratia marcescens | CoA + 6'-N-acetylnetilmicin | - |
? | |
| acetyl-CoA + sisomicin | - |
Serratia marcescens | CoA + 6'-N-acetylsisomicin | - |
? | |
| acetyl-CoA + tobramycin | - |
Serratia marcescens | CoA + 6'-N-acetyltobramycin | - |
? | |
| additional information | enzyme acetylates all the aminoglycosides tested, except for apramycin, gentamicin, and lividomycin | Serratia marcescens | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAC(6')-IaI | - |
Serratia marcescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
amikacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.49 | - |
sisomicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.67 | - |
tobramycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 0.73 | - |
netilmicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 7.8 | - |
neomycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 10 | - |
kanamycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 17 | - |
arbekacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 25 | - |
isepamicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 85 | - |
dibekacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
amikacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 24 | - |
tobramycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 25 | - |
neomycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 32 | - |
netilmicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 32 | - |
kanamycin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 41 | - |
sisomicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 48 | - |
arbekacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 61 | - |
isepamicin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
| 150 | - |
dibekacin | pH not specified in the publication, temperature not specified in the publication | Serratia marcescens | |
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Release 2023.1 (January 2023)
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