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Literature summary for 2.3.1.82 extracted from

  • Smith, C.; Bhattacharya, M.; Toth, M.; Stewart, N.; Vakulenko, S.
    Aminoglycoside resistance profile and structural architecture of the aminoglycoside acetyltransferase AAC(6')-Im (2017), Microb. Cell, 4, 402-410 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.82

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
structure of the kanamycin A complex of AAC(6')-Im. The substrate binds in a shallow positively-charged pocket, with the N6' amino group positioned appropriately for an efficient nucleophilic attack on an acetyl-CoA cofactor. Comparison of fortimicin binding to AAC(6')-Im and AAC(6')-Ie Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli Q93ET8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + amikacin
-
 Escherichia coli CoA + N6'-acetylamikacin
-
 ?
acetyl-CoA + dibekacin
-
 Escherichia coli CoA + N6'-acetyldibekacin
-
 ?
acetyl-CoA + kanamycin A
-
 Escherichia coli CoA + N6'-acetylkanamycin A
-
 ?
acetyl-CoA + kanamycin B
-
 Escherichia coli CoA + N6'-acetylkanamycin B
-
 ?
acetyl-CoA + neomycin
-
 Escherichia coli CoA + N6'-acetylneomycin
-
 ?
acetyl-CoA + tobramycin
-
 Escherichia coli CoA + N6'-acetyltobramycin
-
 ?
additional information strong substrate inhibition with all 4,6-disubstituted aminoglycosides tested at concentrations required to determine kcat and Km values Escherichia coli ?
-
-

Synonyms

Synonyms Comment Organism
6'-acetyltransferase-Im
-
 Escherichia coli
AAC(6')-Im
-
 Escherichia coli

General Information

General Information Comment Organism
physiological function when expressed in the Escherichia coli JM83 strain, AAC(6')-Im produces resistance to a wide range of clinically important 4,6-disubstituted aminoglycosides with minimal inhibitory concentration values 8-128fold above those for the recipient strain Escherichia coli